D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	61,035	A→T	T58T (ACT→ACA)	SAUSA300_RS00265 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	61,035	0	A	T	100.0%	185.7 / NA	67	T58T (ACT→ACA)	SAUSA300_RS00265	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (7/60); total (7/60)

AATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTACCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAA  >  NC_007793/60942‑61131

aataacaaaTATTAATAATTGATGATCAAACGGTTTATCAAAGACTCCTTTTGAAAAAATAAAAATTCCTCTGCCTACACAAAAACTAATCCATGTCata                                                                                            <  1:1696896/94‑1 (MQ=255)

                                                          tgctcttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATcc                                  <  1:1342785/90‑1 (MQ=255)

                                                          tgctcttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTCTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATcc                                  <  1:1447598/90‑1 (MQ=255)

                                                           tcttccgatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCa                                 <  1:2455282/90‑1 (MQ=255)

                                                           gctcatccgaTCTGCCTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCa                                 <  1:220221/91‑1 (MQ=255)

                                                            ttccgatcttCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCAt                                <  1:786688/91‑1 (MQ=255)

                                                             tcttccgaTCTGCCTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATa                               <  1:1142155/93‑1 (MQ=255)

                                                             tccgatcttCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCAtg                               <  1:4467611/92‑2 (MQ=255)

                                                              cttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATAc                              <  1:3360591/94‑1 (MQ=255)

                                                               ttccggtCTGCCTATACAAAAACTAATCCATGTCAGAATATGATTTCCTAAAGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                             <  1:4409056/94‑1 (MQ=255)

                                                               ttccgaTCTGCCTAGACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                             <  1:4724217/95‑1 (MQ=255)

                                                               ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                             <  1:5224148/95‑1 (MQ=255)

                                                               ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                             <  1:2485526/95‑1 (MQ=255)

                                                               ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                             <  1:5716041/95‑1 (MQ=255)

                                                               ccgatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                             <  1:2334305/94‑1 (MQ=255)

                                                                 gatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACaaa                           <  1:2776804/96‑1 (MQ=255)

                                                                    aTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATaa                        <  1:3021366/100‑1 (MQ=255)

                                                                        gcctgcacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCCTTTTTACCACGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                    >  1:312784/9‑100 (MQ=255)

                                                                        gccTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                    <  1:49580/99‑1 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                    >  1:3628319/2‑100 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                    >  1:4033749/2‑100 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                    >  1:3785813/2‑100 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                    <  1:4712518/99‑1 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                    >  1:5478884/2‑100 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGAGTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGGTCATCAGCAAGTATCCATACAAATAAAGGt                    <  1:3935916/99‑1 (MQ=255)

                                                                         cctccacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTa                   <  1:2432831/93‑1 (MQ=255)

                                                                         cctccacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTa                   <  1:5467683/93‑1 (MQ=255)

                                                                          cggcacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGCCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTaa                  <  1:3248254/94‑1 (MQ=255)

                                                                            ataCAAAAACTAATCCATGTCATAATATGATTCCCTAAGGCGTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTagca                <  1:2640796/100‑4 (MQ=255)

                                                                                aaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc            <  1:4624969/100‑1 (MQ=255)

                                                                                aaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc            <  1:5555394/100‑1 (MQ=255)

                                                                                  aaaCTAATCCATGTCATAGTATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCTAGTATCCATACAAATAAAGGTAAAATGACAg          <  1:5038059/98‑1 (MQ=255)

                                                                                    aCTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGt        <  1:2541477/100‑1 (MQ=255)

                                                                                    aCTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGt        <  1:249309/100‑1 (MQ=255)

                                                                                     cTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGta       <  1:5611630/100‑2 (MQ=255)

                                                                                     cTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTg       <  1:4085334/100‑1 (MQ=255)

                                                                                      tAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGc      <  1:377691/100‑1 (MQ=255)

                                                                                      tAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGc      <  1:2022312/100‑1 (MQ=255)

                                                                                       aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATGCAAATAAAGGTAAAATGACAGGTGCg     <  1:2132753/97‑1 (MQ=255)

                                                                                       aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGATAGGTGCg     >  1:2692673/4‑100 (MQ=255)

                                                                                       aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGcgcg     >  1:1708366/4‑100 (MQ=255)

                                                                                       aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGct     <  1:420304/97‑2 (MQ=255)

                                                                                       aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg     <  1:4201379/97‑1 (MQ=255)

                                                                                       aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg     <  1:5503437/97‑1 (MQ=255)

                                                                                       aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg     <  1:5430265/97‑1 (MQ=255)

                                                                                       aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg     <  1:4207953/97‑1 (MQ=255)

                                                                                       aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg     <  1:4386860/97‑1 (MQ=255)

                                                                                       aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg     <  1:3301640/97‑1 (MQ=255)

                                                                                       aatccATGTCATAATATGAGTCCCTAATGCTTTGTTACCATGTCCGGATGTTAGCTCTTCAGCAAGTATCCATACAAATAAAGTTAAAATGACAGGTGCg     <  1:3495627/97‑1 (MQ=255)

                                                                                        atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCgt    <  1:2342875/98‑2 (MQ=255)

                                                                                        atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa    <  1:2855824/98‑1 (MQ=255)

                                                                                        atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa    <  1:2341241/98‑1 (MQ=255)

                                                                                        atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa    <  1:5436286/98‑1 (MQ=255)

                                                                                        atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa    <  1:2345561/98‑1 (MQ=255)

                                                                                        atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa    <  1:5034526/98‑1 (MQ=255)

                                                                                        atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa    <  1:4870723/98‑1 (MQ=255)

                                                                                        atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa    <  1:4822449/98‑1 (MQ=255)

                                                                                        atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa    <  1:1385751/98‑1 (MQ=255)

                                                                                        atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCAGCAGCAAGTATCCATACAAATAAAGGTAAGATGACAGGTGCGa    <  1:2585414/98‑1 (MQ=255)

                                                                                         tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa   <  1:620046/99‑1 (MQ=255)

                                                                                         tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa   <  1:3704873/99‑1 (MQ=255)

                                                                                         tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa   <  1:916508/99‑1 (MQ=255)

                                                                                         tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa   <  1:2565557/99‑1 (MQ=255)

                                                                                         tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa   <  1:3085020/99‑1 (MQ=255)

                                                                                         tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa   <  1:4538667/99‑1 (MQ=255)

                                                                                          ccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaa  <  1:5650104/100‑1 (MQ=255)

                                                                                          ccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaa  <  1:5503416/100‑1 (MQ=255)

AATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTACCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAA  >  NC_007793/60942‑61131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`