Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,589,085	C→G	A310P (GCT→CCT)	SAUSA300_RS13315 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,589,085	0	C	G	100.0%	92.7 / NA	34	A310P (GCT→CCT)	SAUSA300_RS13315	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (22/12);  total (22/12)

TTTCAATTTCTGAAACTTTGCTTACTACAATTTTGAATCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGCATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGATTTTAGCAATTTCTTCGTTTACCTTTTC  >  NC_007793/2588989‑2589175                                                                                                 |                                                                                           tttCAATTTCTGAAACTTTGCTTACTACAATTTTGAATCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGAtt                                                                                         <  1:2115684/100‑1 (MQ=255)   tCAATTTCTGAAACTTTGCTTACTACAATTTTGAATCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTAt                                                                                       <  1:2378462/100‑1 (MQ=255)                  ttGCTTACTACAATTTTGAATCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAAc                                                                        >  1:3781568/1‑100 (MQ=255)                    gCTTACTACAATTTTGAATCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACgg                                                                      >  1:4828563/1‑100 (MQ=255)                      ttACTACAATTTTGAATCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGtt                                                                    <  1:400278/100‑1 (MQ=255)                       tactacAATTTTGAATCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTa                                                                   <  1:971218/100‑1 (MQ=255)                       tactacAATTTTGAATCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTa                                                                   >  1:4235012/1‑100 (MQ=255)                        actacAATTTTGAATCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAGACGAATCAATTCTTCAGGATTATGAGTACATTCGTAACGGCTAg                                                                  >  1:2685246/1‑100 (MQ=255)                                    aaTCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAattgattg                                                      >  1:2527286/1‑100 (MQ=255)                                        ttACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGtttt                                                  >  1:1463486/2‑100 (MQ=255)                                         tACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTta                                                 >  1:1141473/1‑100 (MQ=255)                                          aCTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTtat                                                >  1:4836133/1‑100 (MQ=255)                                                 tttGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTc                                         <  1:1451597/100‑1 (MQ=255)                                                  ttGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCa                                        <  1:3232054/100‑1 (MQ=255)                                                  ttGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCa                                        <  1:28946/100‑1 (MQ=255)                                                         aaTTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTc                                 >  1:4876878/1‑100 (MQ=255)                                                             gctgcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGAt                             >  1:5091603/1‑100 (MQ=255)                                                              ttgcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGAtt                            >  1:25198/2‑100 (MQ=255)                                                              ctgcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGAtt                            >  1:1240213/1‑100 (MQ=255)                                                              ctgcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTCCAACATTTCGGAtt                            >  1:1383757/1‑100 (MQ=255)                                                                gcAACTGATCGACGAAACGAATCAAATCTTCAGGATTGTGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGAtttt                          >  1:5764734/1‑100 (MQ=255)                                                                gcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTCACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGAtttt                          >  1:2494600/1‑100 (MQ=255)                                                                gcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGAtttt                          >  1:2701623/1‑100 (MQ=255)                                                                gcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGAtttt                          >  1:264064/1‑100 (MQ=255)                                                                gcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGAtttt                          <  1:4353514/100‑1 (MQ=255)                                                                gcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGAtttt                          >  1:1994513/1‑100 (MQ=255)                                                                gcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGAtttt                          >  1:770872/1‑100 (MQ=255)                                                                gcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTCCAACATTTCGGAtttt                          >  1:3980082/1‑100 (MQ=255)                                                                gcAACTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGGTAGGTGAATTGATTGTTTTATAAGGTACAACAGATCGGaagag                          >  1:2264170/1‑96 (MQ=255)                                                                    cTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGATTTTAGCa                      <  1:5389183/100‑1 (MQ=255)                                                                    cTGATCGACGAAACGAATCAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACAGATCGGaagagcaca                      >  1:1300080/1‑92 (MQ=255)                                                                                      cAAATCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGATTTTAGCAATTTCTTCGTTTACCttg    <  1:4418563/100‑2 (MQ=255)                                                                                        gaTCTTCAGGCTTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGATTTTAGCAATTTCTTCGTTTACCTTTTc  <  1:243805/99‑1 (MQ=255)                                                                                        aaTCTTCAGGATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGATTTTAGCAATTTCTTCGTTTACCTTTTc  <  1:2811552/100‑1 (MQ=255)                                                                                                 |                                                                                           TTTCAATTTCTGAAACTTTGCTTACTACAATTTTGAATCCTACTGGTTTTTGACCTAATTGCTGCAACTGATCGACGAAACGAATCAAATCTTCAGCATTATGAATAAATTCGTAACGGTTAGGTGAATTGATTGTTTTATAAGGTTCAACATTTCGGATTTTAGCAATTTCTTCGTTTACCTTTTC  >  NC_007793/2588989‑2589175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑