Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,576,724	T→G	I136L (ATA→CTA)	SAUSA300_RS13245 ←	glycine/betaine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,576,724	0	T	G	100.0%	78.1 / NA	30	I136L (ATA→CTA)	SAUSA300_RS13245	glycine/betaine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/20);  total (10/20)

CCAATGCACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATATTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTCCATTTCA  >  NC_007793/2576631‑2576819                                                                                              |                                                                                                ccAATGCACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTctgc                                                                                           <  1:5608105/100‑1 (MQ=255)  cAATGCACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTctgct                                                                                          <  1:1670418/100‑1 (MQ=255)       cACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATa                                                                                     <  1:5530691/100‑1 (MQ=255)       cACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATa                                                                                     <  1:4266375/100‑1 (MQ=255)                             aataatATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGt                                                               <  1:3783297/100‑1 (MQ=255)                                aataTCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTaag                                                            <  1:986384/100‑2 (MQ=255)                                 atatCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTccagatcgg                                                           >  1:1291403/1‑93 (MQ=255)                                 atatCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCgggggca                                                           <  1:2034629/100‑6 (MQ=255)                                      ttGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCcaca                                                      <  1:3491381/100‑1 (MQ=255)                                      ttGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCcaca                                                      <  1:2670992/100‑1 (MQ=255)                                       tGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGttagatcggaa                                                     >  1:101077/1‑90 (MQ=255)                                       tGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACaa                                                     >  1:5310839/1‑100 (MQ=255)                                       tGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACaa                                                     >  1:3714905/1‑100 (MQ=255)                                       tGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGCaaagatcgga                                                     >  1:1636266/1‑93 (MQ=255)                                           tggCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGttt                                                 >  1:2369252/2‑100 (MQ=255)                                                              aacaCCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACgg                              <  1:5748591/100‑2 (MQ=255)                                                                  cccatgcGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTAt                          <  1:604922/94‑1 (MQ=255)                                                                        cGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCttta                    <  1:1479856/100‑2 (MQ=255)                                                                              tgttgCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCctctg              <  1:229652/100‑2 (MQ=255)                                                                              tattgCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCtt              >  1:4087709/3‑100 (MQ=255)                                                                               gttgCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCttt             <  1:1391902/100‑1 (MQ=255)                                                                               gttgCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCttt             <  1:844632/100‑1 (MQ=255)                                                                                  gCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGt          >  1:3930893/1‑100 (MQ=255)                                                                                    ccACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTcc        <  1:1871317/100‑1 (MQ=255)                                                                                    ccACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAAGTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTcc        <  1:2277875/100‑1 (MQ=255)                                                                                      aCCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTCCaa      <  1:685772/100‑2 (MQ=255)                                                                                      aCCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTCCAt      <  1:2099685/100‑1 (MQ=255)                                                                                      aCCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTCCAt      <  1:1906187/100‑1 (MQ=255)                                                                                        atGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTCCAttt    >  1:3052913/2‑100 (MQ=255)                                                                                          gATAGTTCTGCTGGATAACGCTCTAGAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCGTTAgatcggaaga  >  1:2718843/1‑91 (MQ=255)                                                                                              |                                                                                                CCAATGCACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATATTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTCCATTTCA  >  NC_007793/2576631‑2576819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑