Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,576,289	C→T	A281T (GCT→ACT)	SAUSA300_RS13245 ←	glycine/betaine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,576,289	0	C	T	100.0%	46.7 / NA	18	A281T (GCT→ACT)	SAUSA300_RS13245	glycine/betaine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/11);  total (7/11)

ACGTATACCCTGATTTATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGCATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTGATTGGTTTAATCATTACACCTTCTACAGTCTT  >  NC_007793/2576215‑2576385                                                                           |                                                                                                 aCGTATACCCTGATTTATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCataata                                                                         >  1:3359937/1‑100 (MQ=255)          cTGATTTATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAatatagatcgga                                                                >  1:4213349/1‑92 (MQ=255)              tttATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTcca                                                            <  1:4725577/100‑3 (MQ=255)                       tCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTg                                                   >  1:1435227/1‑100 (MQ=255)                       tCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTg                                                   >  1:1929372/1‑100 (MQ=255)                       tCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTg                                                   >  1:5009315/1‑100 (MQ=255)                        cAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGc                                                  <  1:5563453/100‑1 (MQ=255)                         aaTGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCt                                                 <  1:5555973/100‑1 (MQ=255)                          aTGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCtt                                                <  1:4246986/100‑1 (MQ=255)                           tGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTc                                               <  1:97792/100‑1 (MQ=255)                                aaGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCtt                                          <  1:1345133/100‑1 (MQ=255)                                aaGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCta                                          >  1:2579549/1‑99 (MQ=255)                                   aaaCCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGtg                                       <  1:4884207/100‑2 (MQ=255)                                   aaaCCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTa                                       <  1:5154320/100‑1 (MQ=255)                                         aGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCgggc                                 <  1:4308605/100‑4 (MQ=255)                                                ggTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTGATTGGttc                          <  1:4629337/100‑2 (MQ=255)                                                              actacAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCATGTTTGGTTTAATCATTACACCtt            >  1:1157198/1‑100 (MQ=255)                                                                        tAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTGAATGGTTTAATCATTACACCTTCTACAGTCta  <  1:4042958/100‑2 (MQ=255)                                                                           |                                                                                                 ACGTATACCCTGATTTATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGCATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTGATTGGTTTAATCATTACACCTTCTACAGTCTT  >  NC_007793/2576215‑2576385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑