Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,558,380	G→C	intergenic (+117/‑65)	SAUSA300_RS13155 → / → SAUSA300_RS13160	membrane protein/Bcr/CflA family drug resistance efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,558,380	0	G	C	100.0%	17.0 / NA	8	intergenic (+117/‑65)	SAUSA300_RS13155/SAUSA300_RS13160	membrane protein/Bcr/CflA family drug resistance efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (4/4);  total (4/4)

AAACATTAATTCCCTATACTTTATCTGTATCACTACGTTATTCGTGATGATGCATTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAAGTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTAAAGGAGTCTGATCATGCAAT  >  NC_007793/2558286‑2558451                                                                                               |                                                                        aaaCATTAATTCCCTATACTTTATCTGTATCACTACGTTATTCGTGATGATGCATTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAACtacat                                                                    <  1:3055302/100‑1 (MQ=255)    cATTAATTCCCTATACTTTATCTGTATCACTACGTTATTCGTGATGATGCATTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAACtacacacg                                                                 <  1:4193629/100‑2 (MQ=255)                     ttATCTGTATCACTACGTTATTCGTGATGATGCATTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAACTACATACAAATACACACAAAACGta                                                <  1:5704463/100‑1 (MQ=255)                                                 gatgCATTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAACTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTaaa                    >  1:3316190/1‑100 (MQ=255)                                                 gatgCATTAAGAGAATAGGGATTTTTTATATAAACTTGTATTCTAACTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTaaa                    >  1:5591961/1‑100 (MQ=255)                                                  atgCATTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAACTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTAAAg                   >  1:1039479/1‑100 (MQ=255)                                                    gCATTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAACTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTAAAGGa                 >  1:646/1‑100 (MQ=255)                                                                   ggATTTTTTATATAAACTTGTATTCTAACTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTAAAGGAGTCTGATCATGCAAt  <  1:2706014/100‑1 (MQ=255)                                                                                               |                                                                        AAACATTAATTCCCTATACTTTATCTGTATCACTACGTTATTCGTGATGATGCATTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAAGTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTAAAGGAGTCTGATCATGCAAT  >  NC_007793/2558286‑2558451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑