Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 2,534,447 G→A T151M (ACG→ATG)  SAUSA300_RS13020 ← amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,534,4470GA98.8% 241.7 / ‑4.0 84coding (452/720 nt)SAUSA300_RS13020amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (4/79);  minor base . (0/1);  total (4/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

GATGTATCTTTGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCGTTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCTTTCGGTATAGAAATAATACCGCCACGAATAATTTCCGAAGCATA  >  NC_007793/2534360‑2534538
                                                                                       |                                                                                           
gatgTATCTTTGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACg                                                                                 >  1:803203/1‑100 (MQ=255)
  tgTATCTTTGATTAAACTTAAAAATGTATTACCTAGTGCAAGAATCGACACTCGAATTACCTGCAGTAAAATGATACGTTGTATCATTTGACCGTACGTc                                                                               <  1:1414928/100‑1 (MQ=255)
    tATCTTTGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCAt                                                                             <  1:5694663/100‑1 (MQ=255)
    tATCTTTGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCAt                                                                             <  1:4134026/100‑1 (MQ=255)
    tATCTTTGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTCGTATCATTCGACCATACGTCAt                                                                             <  1:492531/100‑1 (MQ=255)
     aTCTTTGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATa                                                                            <  1:4397383/100‑1 (MQ=255)
      atcttGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATag                                                                           <  1:3491721/97‑2 (MQ=255)
        tttGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCg                                                                         <  1:2147632/100‑1 (MQ=255)
        tttGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCg                                                                         <  1:460264/100‑1 (MQ=255)
        tttGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGATAAAATGATACGTTGTATCATTTGACCATACGTCATACCg                                                                         <  1:5063911/100‑1 (MQ=255)
         ttGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGCCATACAGa                                                                        >  1:3547424/1‑100 (MQ=255)
           gATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCacccagatc                                                                      >  1:4744802/1‑92 (MQ=255)
            aTTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATgg                                                                     <  1:3728785/100‑1 (MQ=255)
            aTTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATgg                                                                     <  1:1060767/100‑1 (MQ=255)
            aTTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATgg                                                                     <  1:2273816/100‑1 (MQ=255)
            aTTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATgg                                                                     <  1:2489944/100‑1 (MQ=255)
            aTTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATgg                                                                     <  1:2654588/100‑1 (MQ=255)
            aTTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATgg                                                                     <  1:2682813/100‑1 (MQ=255)
             ttAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGaagca                                                                    <  1:3228284/100‑5 (MQ=255)
             ttAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATggg                                                                    <  1:3448413/100‑2 (MQ=255)
             ttAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGa                                                                    <  1:3773670/100‑1 (MQ=255)
             ttAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGa                                                                    <  1:550407/100‑1 (MQ=255)
             ttAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCAAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGa                                                                    <  1:1511380/100‑1 (MQ=255)
              tAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGag                                                                   <  1:1721129/100‑2 (MQ=255)
               aaaCTTAAAAATGTTTTAACTAGTGCCGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAat                                                                  <  1:3514783/100‑1 (MQ=255)
               aaaCTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAat                                                                  <  1:3998430/100‑1 (MQ=255)
               aaaCTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAat                                                                  <  1:3570768/100‑1 (MQ=255)
               aaaCTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAat                                                                  <  1:5022254/100‑1 (MQ=255)
               aaaCTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAat                                                                  <  1:1172845/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAattgta                                                              <  1:1035549/100‑5 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:2159151/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:1554698/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:4228345/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:4895536/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:4105213/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:4919891/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:1330375/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:1719332/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:3860751/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:1312642/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:5215824/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:3723112/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:3695582/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:5472383/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:1170952/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:5528821/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:5689172/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:1112763/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:763935/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:3264249/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:995388/100‑1 (MQ=255)
                   ttAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGc                                                              <  1:33983/100‑1 (MQ=255)
                    tAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCa                                                             <  1:4766338/100‑1 (MQ=255)
                      aaaaTGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGc                                                           <  1:3427761/100‑1 (MQ=255)
                      aaaaTGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGc                                                           <  1:5028148/100‑1 (MQ=255)
                      aaaaTGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGc                                                           <  1:3661953/100‑1 (MQ=255)
                      aaaaTGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGc                                                           <  1:4052854/100‑1 (MQ=255)
                      aaaaTGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGc                                                           <  1:4045980/100‑1 (MQ=255)
                      aaaaTGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGc                                                           <  1:3806043/100‑1 (MQ=255)
                      aaaaTGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATC‑ATTGACCATACGTCATACCGATGGAATATGCAGCt                                                          <  1:996977/100‑1 (MQ=255)
                       agaTGTAGTACCTCGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGcg                                                          <  1:5778011/98‑2 (MQ=255)
                        aaTGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCtg                                                         <  1:1025714/100‑2 (MQ=255)
                             actACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGtt                                                    >  1:4848122/3‑100 (MQ=255)
                              ttACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGttt                                                   <  1:3106969/100‑1 (MQ=255)
                               tACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTg                                                  <  1:5416551/100‑1 (MQ=255)
                               tACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTg                                                  <  1:5281733/100‑1 (MQ=255)
                                aCCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTgc                                                 <  1:3943042/100‑2 (MQ=255)
                                aCCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGt                                                 <  1:5257762/100‑1 (MQ=255)
                                aCCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGt                                                 <  1:446478/100‑1 (MQ=255)
                                 ccTAGTGCAGGAATTGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTc                                                <  1:178510/100‑1 (MQ=255)
                                  ttAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTcc                                               <  1:3180440/99‑1 (MQ=255)
                                   tAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCt                                              <  1:1600492/100‑1 (MQ=255)
                                   tAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCt                                              <  1:1402772/100‑1 (MQ=255)
                                   tAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCt                                              <  1:441221/100‑1 (MQ=255)
                                    aGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCtt                                             <  1:5197566/100‑1 (MQ=255)
                                    aGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCtt                                             <  1:344488/100‑1 (MQ=255)
                                                           tGCCTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCTTTCGGTATAGAAATAATACCGcag                      <  1:1324739/100‑3 (MQ=255)
                                                              cTGCGGTAAAATGATGCGTTGTATCATTTGACCATACGTCATCCCGATGGAATAGGCAGCTTCTGTTTGTCTTTTCGGTATAGAAATAATACCGCCACGa                   <  1:1569738/100‑1 (MQ=255)
                                                              cTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCTTTCGGTATAGAAATAATACCGCCAcca                   <  1:971337/100‑3 (MQ=255)
                                                              cTGCGGTAAAATGATACGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCTTTCGGTATAGAAATAATACCGCCACGa                   <  1:305563/100‑1 (MQ=255)
                                                                              cGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCTTTCGGTATAGAAATAATACCGCCACGAATAATTTCCGAAGCAt   <  1:2765829/100‑1 (MQ=255)
                                                                              cGTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCTTTCGGTATAGAAATAATACCGCCACGAATAATTTCCGAAGCAt   <  1:962295/100‑1 (MQ=255)
                                                                               gTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCTTTCGGTATAGAAATAATACCGCCACGAATAATTTCCGAAGCAtc  <  1:4522966/100‑2 (MQ=255)
                                                                               gTTGTATCATTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCTTTCGGTATAGAAATAATACCGCCACGAATAATTTCCGAAGCATa  <  1:3449893/100‑1 (MQ=255)
                                                                                       |                                                                                           
GATGTATCTTTGATTAAACTTAAAAATGTATTACCTAGTGCAGGAATCGACACTCGAATTGCCTGCGGTAAAATGATACGTTGTATCGTTTGACCATACGTCATACCGATGGAATATGCAGCTTCTGTTTGTCCTTTCGGTATAGAAATAATACCGCCACGAATAATTTCCGAAGCATA  >  NC_007793/2534360‑2534538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑