D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	61,031	C→T	V60I (GTT→ATT)	SAUSA300_RS00265 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	61,031	0	C	T	100.0%	104.8 / NA	38	V60I (GTT→ATT)	SAUSA300_RS00265	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/33); total (5/33)

AATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTACCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGC  >  NC_007793/60942‑61127

aataacaaaTATTAATAATTGATGATCAAACGGTTTATCAAAGACTCCTTTTGAAAAAATAAAAATTCCTCTGCCTACACAAAAACTAATCCATGTCata                                                                                        <  1:1696896/94‑1 (MQ=255)

                                                          tgctcttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATcc                              <  1:1342785/90‑1 (MQ=255)

                                                          tgctcttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTCTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATcc                              <  1:1447598/90‑1 (MQ=255)

                                                           tcttccgatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCa                             <  1:2455282/90‑1 (MQ=255)

                                                           gctcatccgaTCTGCCTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCa                             <  1:220221/91‑1 (MQ=255)

                                                            ttccgatcttCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCAt                            <  1:786688/91‑1 (MQ=255)

                                                             tcttccgaTCTGCCTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATa                           <  1:1142155/93‑1 (MQ=255)

                                                             tccgatcttCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCAtg                           <  1:4467611/92‑2 (MQ=255)

                                                              cttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATAc                          <  1:3360591/94‑1 (MQ=255)

                                                               ttccggtCTGCCTATACAAAAACTAATCCATGTCAGAATATGATTTCCTAAAGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                         <  1:4409056/94‑1 (MQ=255)

                                                               ttccgaTCTGCCTAGACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                         <  1:4724217/95‑1 (MQ=255)

                                                               ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                         <  1:5716041/95‑1 (MQ=255)

                                                               ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                         <  1:2485526/95‑1 (MQ=255)

                                                               ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                         <  1:5224148/95‑1 (MQ=255)

                                                               ccgatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                         <  1:2334305/94‑1 (MQ=255)

                                                                 gatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACaaa                       <  1:2776804/96‑1 (MQ=255)

                                                                    aTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATaa                    <  1:3021366/100‑1 (MQ=255)

                                                                        gcctgcacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCCTTTTTACCACGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                >  1:312784/9‑100 (MQ=255)

                                                                        gccTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                <  1:49580/99‑1 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                >  1:4033749/2‑100 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                >  1:5478884/2‑100 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                <  1:4712518/99‑1 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                >  1:3785813/2‑100 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                >  1:3628319/2‑100 (MQ=255)

                                                                        gccTACACAAAAACTAATCCATGTCATAATATGAGTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGGTCATCAGCAAGTATCCATACAAATAAAGGt                <  1:3935916/99‑1 (MQ=255)

                                                                         cctccacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTa               <  1:5467683/93‑1 (MQ=255)

                                                                         cctccacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTa               <  1:2432831/93‑1 (MQ=255)

                                                                          cggcacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGCCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTaa              <  1:3248254/94‑1 (MQ=255)

                                                                            ataCAAAAACTAATCCATGTCATAATATGATTCCCTAAGGCGTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTagca            <  1:2640796/100‑4 (MQ=255)

                                                                                aaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc        <  1:4624969/100‑1 (MQ=255)

                                                                                aaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc        <  1:5555394/100‑1 (MQ=255)

                                                                                  aaaCTAATCCATGTCATAGTATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCTAGTATCCATACAAATAAAGGTAAAATGACAg      <  1:5038059/98‑1 (MQ=255)

                                                                                    aCTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGt    <  1:2541477/100‑1 (MQ=255)

                                                                                    aCTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGt    <  1:249309/100‑1 (MQ=255)

                                                                                     cTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGta   <  1:5611630/100‑2 (MQ=255)

                                                                                     cTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTg   <  1:4085334/100‑1 (MQ=255)

                                                                                      tAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGc  <  1:377691/100‑1 (MQ=255)

                                                                                      tAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGc  <  1:2022312/100‑1 (MQ=255)

AATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTACCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGC  >  NC_007793/60942‑61127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`