Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,525,762	C→T	E95K (GAA→AAA)	SAUSA300_RS12960 ←	N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,525,762	0	C	T	96.8%	78.0 / ‑4.6	31	E95K (GAA→AAA)	SAUSA300_RS12960	N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (8/22);  total (8/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

GATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATCGTAGATA  >  NC_007793/2525668‑2525854                                                                                               |                                                                                             gATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTtatagc                                                                                         <  1:1648791/100‑3 (MQ=255)     tACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACGTAAGTAAGTAATTGGCTGCCATAGCCTTTCCCTTTATACTCAgg                                                                                     <  1:1583543/100‑1 (MQ=255)     tACCTAAAGTAAATGCCGCTGACGTTAACGTACCTCGCAATAATGCTTCTTCAACATAAGTAAGTCATTGGCTACCATAGCCTTTCCCTTTATACTCAgg                                                                                     <  1:839011/100‑1 (MQ=255)              tAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCACAGCCTTTCCCTTTATACTCAGGATTTGTCGc                                                                            <  1:2877867/100‑1 (MQ=255)               aaCTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACCCAGGagatcggaag                                                                           >  1:3598175/1‑91 (MQ=255)                aCTGCCGTTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCag                                                                          <  1:1159280/100‑2 (MQ=255)                   gCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAacc                                                                       >  1:653144/1‑100 (MQ=255)                     tacTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAaccac                                                                     >  1:5410466/3‑100 (MQ=255)                      gCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAaccacc                                                                    <  1:1081641/100‑1 (MQ=255)                      gCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAaccacc                                                                    <  1:5422098/100‑1 (MQ=255)                        tGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAg                                                                  <  1:1972818/100‑1 (MQ=255)                           cTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGAcc                                                               <  1:217348/100‑2 (MQ=255)                           cTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGCAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGagg                                                               <  1:4552485/100‑3 (MQ=255)                              tAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACaaaa                                                            <  1:5135086/100‑1 (MQ=255)                                      ctcGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAAcc                                                    <  1:4720223/100‑1 (MQ=255)                                                     cttcAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTcac                                     <  1:1032691/100‑1 (MQ=255)                                                           cATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACttta                               <  1:5052473/100‑3 (MQ=255)                                                             taagtaagTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTcccc                             <  1:1667346/100‑1 (MQ=255)                                                             taagtaagTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTcccc                             <  1:4406829/100‑1 (MQ=255)                                                              aagtaagtAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCa                            <  1:3723471/100‑1 (MQ=255)                                                               agtaagtaATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCag                           <  1:2699653/100‑2 (MQ=255)                                                                gtaagtaaTTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCaat                          <  1:2781539/100‑2 (MQ=255)                                                                 taagtaaTTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCacac                         <  1:4959766/100‑4 (MQ=255)                                                                 taagtaaTTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAgc                         <  1:4094258/100‑2 (MQ=255)                                                                  aagtaaTTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCagcaa                        <  1:4163812/100‑5 (MQ=255)                                                                         tGGCTACCATAGCCTTTCCCTTCATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTag                 <  1:2268257/100‑2 (MQ=255)                                                                           tcTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAAcc               >  1:2331270/2‑100 (MQ=255)                                                                              aCCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTa            >  1:2864980/1‑100 (MQ=255)                                                                                 ttAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATc         >  1:5217532/2‑100 (MQ=255)                                                                                 aTAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATc         >  1:5001314/1‑100 (MQ=255)                                                                                     ccTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATCGtgg     <  1:2181728/100‑3 (MQ=255)                                                                                        ttCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATCGTAGata  >  1:184100/1‑100 (MQ=255)                                                                                               |                                                                                             GATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATCGTAGATA  >  NC_007793/2525668‑2525854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑