Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,515,965	C→A	V280F (GTT→TTT)	narH ←	nitrate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,515,965	0	C	A	100.0%	56.9 / NA	21	V280F (GTT→TTT)	narH	nitrate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/15);  total (6/15)

GCAATGACTTCTTCGTCAAATGGGTTTAAGAATATATCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAACTCGATCTGCATCATATAATAAAACACCTAAGTAACGCATACGTCCTGTACAAGTTTCAGAGCATACCGTAGGCATACCCGCCT  >  NC_007793/2515874‑2516048                                                                                            |                                                                                    gCAATGACTTCTTCGTCAAATGGGTTTAAGAATATATCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTg                                                                             <  1:4544199/100‑1 (MQ=255)  cAATGACTTCTTCGTCAAATGGGTTTAAGAATATATCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGc                                                                            <  1:2408619/100‑1 (MQ=255)  cAATGACTTCTTCGTCAAATGGGTTTAAGAATATATCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGc                                                                            <  1:3149250/100‑1 (MQ=255)   aaTGACTTCTTCGTCAAATGGGTTTAAGAATATATCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGca                                                                           <  1:3159867/100‑1 (MQ=255)     tGACTTCTTCGTCAAATGGGTTTAAGAATATATCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGcatc                                                                         <  1:889138/100‑1 (MQ=255)     tGACTTCTTCGTCAAATGGGTTTAAGAATATATCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGcatc                                                                         <  1:477918/100‑1 (MQ=255)             tcGTCAAATGGGTTTAAGAATATATCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATataata                                                                 >  1:1878878/1‑100 (MQ=255)                             aGAATATATCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACg                                                 >  1:5687106/1‑100 (MQ=255)                                atatatCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACGCAt                                              <  1:1627295/100‑1 (MQ=255)                                atatatCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACGCAt                                              <  1:4256694/100‑1 (MQ=255)                                atatatCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACGCAt                                              <  1:4224133/100‑1 (MQ=255)                                  atatCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACGCAtgc                                            <  1:2957120/100‑3 (MQ=255)                                        aaTTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACGCATACGTCCTg                                      >  1:4088458/1‑100 (MQ=255)                                        aaTTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACGCATACGTCCTg                                      >  1:3079723/1‑100 (MQ=255)                                        aaTTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACGCATACGTCCTg                                      <  1:4985558/100‑1 (MQ=255)                                               gctCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACGCATACGTCCTGTACAAGt                               >  1:365938/3‑100 (MQ=255)                                                    tataAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACGCATACGTCCTGTACAAGTTTCag                          <  1:2492060/100‑1 (MQ=255)                                                              tctcGTCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAGCACCTAAGTAACGCATACGTCCTGTACAAGTTTCAGAGCATACCGt                <  1:5231904/100‑1 (MQ=255)                                                                  atCTACTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACTCCTAAGTAACGCATACGTCCTGTACAAGTTTCAGAGCATACCGTAGGc            <  1:5396597/99‑1 (MQ=255)                                                                      aCTGCTGAAGCTGCTTCATGAAATCGATCTGCATCATATAATAAAACACCTAAGTAACGCATACGTCCTGTACAAGTTTCAGAGCATACCGTAGGCATAc        >  1:378486/1‑100 (MQ=255)                                                                            gAAGCGGCTTCATGAAATCGATCTCCATCATATAATAAAACTCCTAATTAACGCATACGTCCTGTACAAGTTTCAGAGCATACCGTAGGCATACCCGcca  <  1:1345302/100‑2 (MQ=255)                                                                                            |                                                                                    GCAATGACTTCTTCGTCAAATGGGTTTAAGAATATATCTAATTGTTTTTCATATAAATCTTTCTCGTCTACTGCTGAAGCTGCTTCATGAACTCGATCTGCATCATATAATAAAACACCTAAGTAACGCATACGTCCTGTACAAGTTTCAGAGCATACCGTAGGCATACCCGCCT  >  NC_007793/2515874‑2516048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑