Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,508,758	T→C	I261V (ATT→GTT)	SAUSA300_RS12890 ←	nitrate transporter NarT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,508,758	0	T	C	100.0%	46.1 / NA	19	I261V (ATT→GTT)	SAUSA300_RS12890	nitrate transporter NarT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/9);  total (10/9)

TATGGTCTGAAATACCTAAAATAATGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAATGCCACCTATTGGTCTTAAGAATGTTGCCAGCGCAATGAATACACCTGATCGAATACCAGCATCTACTTTGTCAATTCCAAAATGATTTACTA  >  NC_007793/2508665‑2508850                                                                                              |                                                                                             tctGGTCTGAAATACCTAAAATAATGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCAct                                                                                        <  1:2287835/98‑2 (MQ=255)    ggTCTGAAATACCTAAAATAATGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTaa                                                                                     <  1:4086466/100‑2 (MQ=255)      tCTGAAATACCTAAAATAATGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTaaag                                                                                   <  1:4921088/100‑4 (MQ=255)         gAAATACCTAAAATAATGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCAccagatcgga                                                                                >  1:5680965/1‑92 (MQ=255)         gAAATACCTAAAATAATGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTc                                                                                >  1:1845806/1‑100 (MQ=255)         gAAATACCTAAAATAATGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTc                                                                                >  1:1243525/1‑100 (MQ=255)                       catGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGcc                                                                  >  1:5578776/2‑100 (MQ=255)                        ttGGCACCGATAATCATAGCAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCa                                                                 >  1:5374142/2‑100 (MQ=255)                        ctGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCa                                                                 >  1:1780116/2‑100 (MQ=255)                        atGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCa                                                                 >  1:5671936/1‑100 (MQ=255)                         tGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCAg                                                                >  1:2769473/1‑100 (MQ=255)                            ggccGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCAgcgc                                                             >  1:2395846/3‑100 (MQ=255)                                         aacaacAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCAGCGCAATGAATACAcca                                                >  1:661685/1‑99 (MQ=255)                                                    caatcaTCAATACTTTAACTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCAGCGCAATGAATACACCTGATCGAATAcc                                     <  1:3151142/100‑1 (MQ=255)                                                                    aaCTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCAGCGCAATGAATACACCTGATCGAATACCAGCATCTACTTTGTCa                     <  1:522825/100‑1 (MQ=255)                                                                    aaCTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCAGCGCAATGAATACACCTGATCGAATACCAGCATCTACTTTGTCa                     <  1:480604/100‑1 (MQ=255)                                                                     aCTGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCAGCGCAATGAATACACCTGATCGAATACCAGCATCTACTTTGTCaa                    <  1:1387058/100‑1 (MQ=255)                                                                       tGCATTAAATTTATCACCTAAAACGCCACCTATTGGTCTTAAGAATGTTGCCAGCGCAATGAATACACCTGATCGAATACCAGCATCTACTTTGTCAAtt                  <  1:1425744/100‑1 (MQ=255)                                                                                       ccTAAAACGCCTCCTATTGGTCTTAAGAATGTTGCCAGCGCAATGAATACACCTGATCGAATACCAGCATCTACTTTGTCAATTCCAAAATGATTTACTa  <  1:2865405/100‑1 (MQ=255)                                                                                              |                                                                                             TATGGTCTGAAATACCTAAAATAATGGCACCGATAATCATAACAACAAAATCAATCATCAATACTTTAACTGCATTAAATTTATCACCTAAAATGCCACCTATTGGTCTTAAGAATGTTGCCAGCGCAATGAATACACCTGATCGAATACCAGCATCTACTTTGTCAATTCCAAAATGATTTACTA  >  NC_007793/2508665‑2508850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑