Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,496,840	C→T	H14Y (CAT→TAT)	SAUSA300_RS12840 →	magnesium transporter CorA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,496,840	0	C	T	100.0%	161.5 / NA	55	H14Y (CAT→TAT)	SAUSA300_RS12840	magnesium transporter CorA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/5);  total (50/5)

TCACTAACTTAAGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAACATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGAACCAGACCGAGAAGAAATTGAAAATCTTATGGAACAATAT  >  NC_007793/2496744‑2496932                                                                                                 |                                                                                             tCACTAACTTAAGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAAtata                                                                                           >  1:1087441/1‑100 (MQ=255)  cACTAACTTAAGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAatatat                                                                                          >  1:3171542/1‑100 (MQ=255)  cACTAACTTAAGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAatatat                                                                                          >  1:3176489/1‑100 (MQ=255)  cACTAACTTAAGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAatatat                                                                                          >  1:837530/1‑100 (MQ=255)  cACTAACTTAAGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAatatat                                                                                          >  1:356689/1‑100 (MQ=255)  cACTAACTTAAGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAatatat                                                                                          >  1:392800/1‑100 (MQ=255)  cACTAACTTAAGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCACTCAGACATTCTGAAGATATAGATAAatatat                                                                                          >  1:314506/1‑100 (MQ=255)      aaCTTAAGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATtata                                                                                      >  1:4184569/1‑100 (MQ=255)          gtggTAATACTCGC‑TTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAac                                                                                 >  1:290800/4‑100 (MQ=255)            aGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCACTCAGACATTCTGAAGATATAGATAAATATATTATAAAAaca                                                                                >  1:1347186/1‑100 (MQ=255)             ttAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAacac                                                                               >  1:4213680/2‑100 (MQ=255)               aaTACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCa                                                                             >  1:3326312/1‑100 (MQ=255)                ttACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCAt                                                                            >  1:129118/2‑100 (MQ=255)                aTACCCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACAccgt                                                                            <  1:5765672/100‑3 (MQ=255)                 tACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATGTATTATAAAAACACCAtt                                                                           <  1:4908147/100‑1 (MQ=255)                 tACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCact                                                                           >  1:4755411/1‑98 (MQ=255)                 tACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCAtt                                                                           <  1:2259296/100‑1 (MQ=255)                 ctcTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGAAAAATATATTATAAAAACACCAtt                                                                           >  1:1089485/3‑100 (MQ=255)                 caCTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCAtt                                                                           >  1:3016682/2‑100 (MQ=255)                  aCTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCacag                                                                          >  1:442613/1‑97 (MQ=255)                  aCTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTa                                                                          >  1:3406218/1‑100 (MQ=255)                  aCTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTa                                                                          >  1:4080966/1‑100 (MQ=255)                  aCTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTa                                                                          >  1:1064584/1‑100 (MQ=255)                  aCTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTa                                                                          >  1:2015625/1‑100 (MQ=255)                  aCTCGCTTTTCCTAAATCCCTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTa                                                                          >  1:5185113/1‑100 (MQ=255)                   cTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAg                                                                         >  1:5350527/1‑100 (MQ=255)                     cGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACGCCATTAGAt                                                                       >  1:2421903/1‑100 (MQ=255)                     cGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGAt                                                                       >  1:1389086/1‑100 (MQ=255)                     cGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGAt                                                                       >  1:1720601/1‑100 (MQ=255)                     cGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGAt                                                                       >  1:1470820/1‑100 (MQ=255)                     cGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGAt                                                                       >  1:3863610/1‑100 (MQ=255)                     cGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGAt                                                                       >  1:3248550/1‑100 (MQ=255)                     cGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGAt                                                                       >  1:597136/1‑100 (MQ=255)                     cGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGAt                                                                       >  1:2948779/1‑100 (MQ=255)                            ccTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAg                                                                >  1:3226614/1‑100 (MQ=255)                            ccTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATAAGATcggaaga                                                                >  1:2380650/1‑94 (MQ=255)                                aaTCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTc                                                            >  1:4928294/1‑100 (MQ=255)                                aaTCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTc                                                            >  1:4318479/1‑100 (MQ=255)                                    gttgggAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATgg                                                        >  1:2100644/6‑100 (MQ=255)                                    cTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATgg                                                        >  1:554989/1‑100 (MQ=255)                                       tAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGAtt                                                     >  1:535639/1‑100 (MQ=255)                                        aGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTa                                                    >  1:1413639/1‑100 (MQ=255)                                        aGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTa                                                    >  1:2976851/1‑100 (MQ=255)                                        aGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTa                                                    >  1:3117723/1‑100 (MQ=255)                                          aaaGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAAt                                                  >  1:2261274/1‑100 (MQ=255)                                            aGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATgt                                                >  1:58007/1‑100 (MQ=255)                                             agAACTATATATCATGATTACTTCATTCGGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATgta                                               >  1:4626836/2‑100 (MQ=255)                                              tgaCTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATgtag                                              >  1:2879223/3‑100 (MQ=255)                                              gAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATgtag                                              >  1:5309136/1‑100 (MQ=255)                                               caCTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATgtagt                                             >  1:899893/2‑100 (MQ=255)                                                   atatatCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTGGaa                                         >  1:5081348/1‑100 (MQ=255)                                                   atatatCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGaa                                         >  1:149046/1‑100 (MQ=255)                                                     atatCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAGTATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGAAcc                                       <  1:1640109/100‑1 (MQ=255)                                                     atatCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGAAcc                                       <  1:3427480/100‑1 (MQ=255)                                                         cATGATTACTTCATTCAGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGAACCAGat                                   >  1:399160/1‑99 (MQ=255)                                                                   tcattcaGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGAACCAGATCg‑gaagagca                        >  1:3667442/1‑97 (MQ=255)                                                                      tacaGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGAACCAGATCg‑gaagagcacac                     >  1:4068296/3‑94 (MQ=255)                                                                       acaGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGAGCCAGATCg‑gaagagcacacg                    >  1:4410952/2‑93 (MQ=255)                                                                        caGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTATAACCAGATCg‑gaagagcacacgt                   >  1:3291461/1‑92 (MQ=255)                                                                        caGACATTCTGAAGATATAGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAAAACCAGATCg‑gaagagcacacgt                   >  1:2874794/1‑92 (MQ=255)                                                                                  gAAGATATAAATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGAACCAGACCGAGAAGAAATTGAAAATCTTATgg          >  1:4170037/1‑100 (MQ=255)                                                                                          aGATAAATATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGAACCAGACCGAGAAGAAATTGAAAATCTTATGGAACaatat  >  1:4123583/1‑100 (MQ=255)                                                                                                 |                                                                                             TCACTAACTTAAGTAATACTCGCTTTTCCTAAATCCTTTAGAAAGGAACTATATATCATGATTACTTCATTCAGACATTCTGAAGATATAGATAAACATATTATAAAAACACCATTAGATCATACAGCGTCATGGATTAATGTAGTAGAACCAGACCGAGAAGAAATTGAAAATCTTATGGAACAATAT  >  NC_007793/2496744‑2496932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑