Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	61,025	G→A	L62F (CTT→TTT)	SAUSA300_RS00265 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	61,025	0	G	A	100.0%	68.2 / NA	25	L62F (CTT→TTT)	SAUSA300_RS00265	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/21);  total (4/21)

AATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTACCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAC  >  NC_007793/60942‑61121                                                                                    |                                                                                                 aataacaaaTATTAATAATTGATGATCAAACGGTTTATCAAAGACTCCTTTTGAAAAAATAAAAATTCCTCTGCCTACACAAAAACTAATCCATGTCata                                                                                  <  1:1696896/94‑1 (MQ=255)                                                           tgctcttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATcc                        <  1:1342785/90‑1 (MQ=255)                                                           tgctcttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTCTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATcc                        <  1:1447598/90‑1 (MQ=255)                                                            tcttccgatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCa                       <  1:2455282/90‑1 (MQ=255)                                                            gctcatccgaTCTGCCTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCa                       <  1:220221/91‑1 (MQ=255)                                                             ttccgatcttCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCAt                      <  1:786688/91‑1 (MQ=255)                                                              tcttccgaTCTGCCTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATa                     <  1:1142155/93‑1 (MQ=255)                                                              tccgatcttCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCAtg                     <  1:4467611/92‑2 (MQ=255)                                                               cttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATAc                    <  1:3360591/94‑1 (MQ=255)                                                                ttccggtCTGCCTATACAAAAACTAATCCATGTCAGAATATGATTTCCTAAAGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                   <  1:4409056/94‑1 (MQ=255)                                                                ttccgaTCTGCCTAGACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                   <  1:4724217/95‑1 (MQ=255)                                                                ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                   <  1:2485526/95‑1 (MQ=255)                                                                ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                   <  1:5716041/95‑1 (MQ=255)                                                                ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                   <  1:5224148/95‑1 (MQ=255)                                                                ccgatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                   <  1:2334305/94‑1 (MQ=255)                                                                  gatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACaaa                 <  1:2776804/96‑1 (MQ=255)                                                                     aTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATaa              <  1:3021366/100‑1 (MQ=255)                                                                         gcctgcacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCCTTTTTACCACGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:312784/9‑100 (MQ=255)                                                                         gccTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt          <  1:49580/99‑1 (MQ=255)                                                                         gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:3628319/2‑100 (MQ=255)                                                                         gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:3785813/2‑100 (MQ=255)                                                                         gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:4033749/2‑100 (MQ=255)                                                                         gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt          <  1:4712518/99‑1 (MQ=255)                                                                         gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:5478884/2‑100 (MQ=255)                                                                         gccTACACAAAAACTAATCCATGTCATAATATGAGTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGGTCATCAGCAAGTATCCATACAAATAAAGGt          <  1:3935916/99‑1 (MQ=255)                                                                          cctccacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTa         <  1:5467683/93‑1 (MQ=255)                                                                          cctccacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTa         <  1:2432831/93‑1 (MQ=255)                                                                           cggcacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGCCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTaa        <  1:3248254/94‑1 (MQ=255)                                                                             ataCAAAAACTAATCCATGTCATAATATGATTCCCTAAGGCGTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTagca      <  1:2640796/100‑4 (MQ=255)                                                                                 aaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc  <  1:4624969/100‑1 (MQ=255)                                                                                 aaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc  <  1:5555394/100‑1 (MQ=255)                                                                                    |                                                                                                 AATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTACCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAC  >  NC_007793/60942‑61121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑