Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 2,496,387 G→T A86S (GCG→TCG)  SAUSA300_RS12835 → TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,496,3870GT99.0% 305.1 / ‑4.4 104A86S (GCG→TCG) SAUSA300_RS12835TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (103/0);  total (104/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 104 total aligned reads displayed.

ATATGTTACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTGCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATAATTTAGAGTTTTATCATACTATTTTACAATTGGAACG  >  NC_007793/2496292‑2496478
                                                                                               |                                                                                           
atatGTTACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGaa                                                                                         >  1:3809792/1‑100 (MQ=255)
    cttACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACt                                                                                     >  1:2479206/2‑100 (MQ=255)
    cctACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACt                                                                                     >  1:155208/3‑100 (MQ=255)
     ttACTCGCAGATATGGAAGATGAGTATATTTCGGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtt                                                                                    >  1:445880/1‑100 (MQ=255)
     ttACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtt                                                                                    >  1:4656477/1‑100 (MQ=255)
     ctACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtt                                                                                    >  1:4948124/2‑100 (MQ=255)
     ctACTCGCAGATATGGAAGATGAGTATATTCCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtt                                                                                    >  1:3531130/2‑100 (MQ=255)
     atACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtt                                                                                    >  1:473814/2‑100 (MQ=255)
      tACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGGCATTTCGAATACttt                                                                                   >  1:1810042/1‑100 (MQ=255)
      tACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACttt                                                                                   >  1:4476200/1‑100 (MQ=255)
      tACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACttt                                                                                   >  1:2696705/1‑100 (MQ=255)
      tACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACttt                                                                                   >  1:5497632/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:689651/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:861686/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:44064/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:3755858/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:3403081/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:332878/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:4657311/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:4346560/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:295232/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:3871240/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:89611/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:3996003/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:4025829/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:957542/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:1330991/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:137845/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:939785/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:24059/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:5205128/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:1683089/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:5249761/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:5576973/1‑100 (MQ=255)
       aCTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:2678357/1‑100 (MQ=255)
       aCTCGCAGATATGAAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGATCTTCAATTGAAGACATTTCGAATACtttt                                                                                  >  1:779897/1‑100 (MQ=255)
        cTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTg                                                                                 >  1:5561212/1‑100 (MQ=255)
         tCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGt                                                                                >  1:1349137/1‑100 (MQ=255)
         gtgCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGt                                                                                >  1:924105/3‑100 (MQ=255)
         ccGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGt                                                                                >  1:2469342/2‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:5519005/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:458601/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:5313574/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:5240804/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:4401460/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:4855169/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:5109586/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:5623511/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:776020/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:4184535/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:501642/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:2363217/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:1986539/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:3471142/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:1960995/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:1868488/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:3526618/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:3600420/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:3686461/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTa                                                                               >  1:1237931/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATCGAAGACATTTCGAATACTTTTGTa                                                                               >  1:2339087/1‑100 (MQ=255)
          cGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGCTCTTCAATTGAATACATTTCGAATACTTTTGTa                                                                               >  1:5660404/1‑100 (MQ=255)
            gtgctatGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTaaa                                                                             >  1:2698426/5‑100 (MQ=255)
            cAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTaaa                                                                             >  1:256216/1‑100 (MQ=255)
            cAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTaaa                                                                             >  1:2063780/1‑100 (MQ=255)
              tctatGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAaata                                                                           >  1:4876039/3‑100 (MQ=255)
                   ggAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAAtata                                                                      >  1:4824285/1‑100 (MQ=255)
                                    cAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCa                                                     >  1:855198/1‑100 (MQ=255)
                                          tAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACatat                                               >  1:3315697/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACacaga                                              >  1:5079262/1‑96 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:2320815/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:5389724/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:4260231/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:93010/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:577157/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:2502999/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:874863/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:4851831/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:4828710/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:480162/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:3307230/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:3473644/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:4547142/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:3793208/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:4328822/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:4149637/1‑100 (MQ=255)
                                           aaCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTGCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:4333885/1‑100 (MQ=255)
                                           aaCGACACATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGCAAATAATATACTCAAAAATATTTTCCAACATAtt                                              >  1:1081341/1‑100 (MQ=255)
                                            aCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTc                                             >  1:5464890/1‑100 (MQ=255)
                                            aCGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTc                                             >  1:740161/1‑100 (MQ=255)
                                                   ataCTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATaa                                      >  1:121867/1‑100 (MQ=255)
                                                   ataCTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATaa                                      >  1:1289754/1‑100 (MQ=255)
                                                     aCTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATAAtt                                    >  1:2775016/1‑100 (MQ=255)
                                                        cAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATAATTTag                                 >  1:359238/1‑100 (MQ=255)
                                                           cttgatttgTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATAATTTAGAGt                              >  1:1578520/2‑100 (MQ=255)
                                                              gatttgTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATAATTTAGAGtttt                           >  1:3499658/1‑100 (MQ=255)
                                                                  tgTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATAATTTAGAGTTTTATCa                       >  1:4175985/1‑100 (MQ=255)
                                                                  tgTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATAATTTAGAGTTTTATCa                       >  1:2512602/1‑100 (MQ=255)
                                                                   gTTACGCGGTTCTTCAATTGAAGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATAATTTAGAGTTTTATCAt                      >  1:2913259/1‑100 (MQ=255)
                                                                                       aaGACATTTCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATAATTTAGAGTTTTATCATACTATTTTACAATTGGAACg  >  1:1832217/1‑100 (MQ=255)
                                                                                               |                                                                                           
ATATGTTACTCGCAGATATGGAAGATGAGTATATTTCAGAACTAACGACATATACTCAATTTGATTTGTTACGCGGTTCTTCAATTGAAGACATTGCGAATACTTTTGTAAATAATATACTCAAAAATATTTTCCAACATATTCATGATAATTTAGAGTTTTATCATACTATTTTACAATTGGAACG  >  NC_007793/2496292‑2496478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑