Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,469,973	T→C	H571R (CAT→CGT)	SAUSA300_RS12695 ←	multidrug MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,469,973	0	T	C	100.0%	88.6 / NA	35	H571R (CAT→CGT)	SAUSA300_RS12695	multidrug MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/28);  total (7/28)

TGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTA  >  NC_007793/2469900‑2470066                                                                          |                                                                                              tGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTc                                                                     >  1:2993150/1‑100 (MQ=255) tGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTc                                                                     >  1:4879687/1‑100 (MQ=255) tGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTc                                                                     >  1:4884054/1‑100 (MQ=255)               cAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAActg                                                       <  1:4051163/100‑2 (MQ=255)                     gtagtaACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTcccc                                                 <  1:4454924/100‑1 (MQ=255)                       agtaACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCaa                                               <  1:5768609/100‑1 (MQ=255)                        gtaACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCaag                                              <  1:1145534/100‑2 (MQ=255)                        gtaACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCaaa                                              <  1:5771015/100‑1 (MQ=255)                        gtaACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCaaa                                              <  1:4104370/100‑1 (MQ=255)                             acTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCGCGCATATGATATTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTg                                         <  1:5780764/100‑1 (MQ=255)                             acTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTg                                         <  1:453040/100‑1 (MQ=255)                             acTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTg                                         <  1:3356200/100‑1 (MQ=255)                               tttCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGag                                       <  1:2354416/100‑2 (MQ=255)                                ttCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATa                                      <  1:656476/100‑1 (MQ=255)                                ttCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATa                                      <  1:3540169/100‑1 (MQ=255)                                ttCATTGCGCCTTCTTGCCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATa                                      <  1:1713449/100‑1 (MQ=255)                                 tCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATaa                                     <  1:328398/100‑1 (MQ=255)                                          cttcttGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTgtgt                            <  1:2195052/100‑1 (MQ=255)                                              cgatctgCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGtt                        <  1:3367200/94‑1 (MQ=255)                                               tgttagCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGttt                       >  1:2930728/6‑100 (MQ=255)                                               tGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGttt                       >  1:261169/1‑100 (MQ=255)                                                 tCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTtgt                     <  1:1984462/100‑1 (MQ=255)                                                  ccGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTcgtg                    >  1:4351152/1‑100 (MQ=255)                                                      cctatTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGtgttgg                <  1:4570465/98‑2 (MQ=255)                                                         gttGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCAt             <  1:2062733/99‑1 (MQ=255)                                                            gtgATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTCTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTAc          >  1:2919779/1‑100 (MQ=255)                                                               aTGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGt       <  1:124509/100‑1 (MQ=255)                                                               aTGCTAATTCACGCATATGATCTCGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGt       <  1:1124296/100‑1 (MQ=255)                                                                tGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGtg      <  1:2618616/100‑2 (MQ=255)                                                                    aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTATCTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:4181569/100‑1 (MQ=255)                                                                    aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:3714217/100‑1 (MQ=255)                                                                    aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:4251776/100‑1 (MQ=255)                                                                    aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:2851862/100‑1 (MQ=255)                                                                    aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:787727/100‑1 (MQ=255)                                                                    aaTTCACGCATATGATCTTGTACAACAGGATTCCTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:3299488/100‑1 (MQ=255)                                                                          |                                                                                              TGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTA  >  NC_007793/2469900‑2470066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑