Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,452,005	T→C	N193N (AAT→AAC)	SAUSA300_RS12595 →	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,452,005	0	T	C	100.0%	24.6 / NA	11	N193N (AAT→AAC)	SAUSA300_RS12595	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/1);  total (10/1)

TTATTAGCACCCAAGGAAACATTTAAAAATCAAAATTGGGTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAATTCTCAAGTGCGCAAACACTTAGATGACTATTTTAATAGAAGAAATATTCGTCCAAATGTCGTTGTAGAAACAGATCGATTCGAATC  >  NC_007793/2451925‑2452091                                                                                 |                                                                                       ttattaGCACCCAAGGAAACATTTAAAAATCAAAATTGGGTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAACTCTCAAGTGCGCAAACACt                                                                     >  1:4879564/1‑100 (MQ=255)   ctgggCACCCAAGGAAACATTTAAAAATCAAAATTGGGTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAACTCTCAAGTGCGCAAACACTTa                                                                   >  1:4169870/5‑100 (MQ=255)     caGCACCCAAGGAAACATTTAAAAATCAAATTTGGGTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAACTCTCAAGTGCGCAAACACTTAGa                                                                 >  1:150079/2‑100 (MQ=255)       gCACCCAGGGAAACATTTAAAAATCAAAATTGGGTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAACTCTCAAGTGCGCAAACACTTAGATg                                                               >  1:4727099/1‑100 (MQ=255)       gCACCCAAGGAAACATTTAAAAATCAAAATTGGGTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAACTCTCAAGTGCGCAAACACTTAGATg                                                               >  1:2540210/1‑100 (MQ=255)                gAAACATTTAAAAATCAAAATTGGGTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAACTCTCAAGTGCGCAAACACTTAGATGACTATTTTa                                                      >  1:559380/1‑100 (MQ=255)                           aaaTCAAAATTGGGTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAACTCTCAAGTGCGCAAACACTTAGATGACTATTTTAATAGAAGAAat                                           >  1:4727363/1‑100 (MQ=255)                                     tGGGTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAACTCTCAAGTGCGCAAACACTTAGATGACTATTTTAATAGAAGAAATATTCGTCCaa                                 >  1:3667536/1‑100 (MQ=255)                                      gggTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAACTCTCAAGTGCGCAAACACTTAGATGACTATTTTAATAGAAGAAATATTCGTCCaaa                                >  1:2509511/1‑100 (MQ=255)                                                     aTTTGCCACTCATATTACCAAACAAAAACTCTCAAGTGCGCAAACACTTAGATGACTATTTTAATAGAAGAAATATTCGTCCAAATGTCGTTGTAGAAAc                 <  1:603222/100‑1 (MQ=255)                                                                    tACCAAACAAAAACTCTCAAGTGCGCAAACACTTAGATGACTATTTTAATAGAAGAAATATTCGTCCAAATGTCGTTGTAGAAACAGATCGATTCGaaac  >  1:1085519/1‑98 (MQ=255)                                                                                 |                                                                                       TTATTAGCACCCAAGGAAACATTTAAAAATCAAAATTGGGTAGATGTTGAAAATTTGCCACTCATATTACCAAACAAAAATTCTCAAGTGCGCAAACACTTAGATGACTATTTTAATAGAAGAAATATTCGTCCAAATGTCGTTGTAGAAACAGATCGATTCGAATC  >  NC_007793/2451925‑2452091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑