Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,447,261	G→A	I277I (ATC→ATT)	SAUSA300_RS12580 ←	N‑acetyl‑L,L‑diaminopimelate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,447,261	0	G	A	100.0%	15.2 / NA	7	I277I (ATC→ATT)	SAUSA300_RS12580	N‑acetyl‑L,L‑diaminopimelate deacetylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/7);  total (0/7)

GGCTACTGCAATTGCTTTATGCGTCAATGCCTCATCATTCATCACAGCGCCAGGTAAATGCGTATAATTTAAATTGATTTTCATATTATATGCTTGAGCCAATCCGTCCGCAATATCTTGTAATCGTGTTTCTACAAGCTTTCGTACCACAGGATCAAA  >  NC_007793/2447186‑2447344                                                                            |                                                                                    ggCTACTGCAATTGCTTTATGCGTCAATGCCTCATCATTCATCACAGCGCCAGGTAAATGCGTATAATTTAAATTAATTTTCATATTATATGCTTGAGcc                                                             <  1:910175/100‑1 (MQ=255)           aTTGCTTTATGCGTCAATGCCTCATCATTCATCACAGCGCCAGGTAAATGCGTATAATTTAAATTAATTTTCATATTATATGCTTGAGCCAAtccgtccg                                                   <  1:496951/100‑1 (MQ=255)            ttGCTTTATGCGTCAATGCCTCATCATTCATCACAGCGCCAGGTAAATGCGTATAATTTAAATTAATTTTCATATTATATGCTTGAGCCAATCCGTCCGc                                                  <  1:4682622/100‑1 (MQ=255)                                            acaGCGCCAGGTAAATGCGTATAATTTAAATTAATTTTCATATTATATGCTTGAGCCAATCCGTCCGCAATATCTTGTAATCGTGTTTCTACAAGCtttg                  <  1:5146096/100‑2 (MQ=255)                                                 gcCAGGTAAATGCGTATAATTTAAATTAATTTTCATATTATATGCTTGAGCCAATCCGTCCGCAATATCTTGTAATCGTGTTTCTACAAGCTTTCGTAcc             <  1:4260112/100‑1 (MQ=255)                                                          aTGCATATAATTTAAATTAATTTTCATATTATATGCTTGAGCCAATCCGTCCGCAATATCTTGTAATCGTGTTTCTACAAGCTTTCGTACCACAggcggc    <  1:2684602/100‑5 (MQ=255)                                                            gCGTATAATTTAAATTAATTTTCATATTATATGCTTGAGCCAATCCGTCCGCAATATCTTGTAATCGTGTTTCTACAAGCTTTCGTACCACAGGATCaac  <  1:204994/100‑2 (MQ=255)                                                                            |                                                                                    GGCTACTGCAATTGCTTTATGCGTCAATGCCTCATCATTCATCACAGCGCCAGGTAAATGCGTATAATTTAAATTGATTTTCATATTATATGCTTGAGCCAATCCGTCCGCAATATCTTGTAATCGTGTTTCTACAAGCTTTCGTACCACAGGATCAAA  >  NC_007793/2447186‑2447344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑