Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,445,965	G→A	intergenic (‑474/+13)	SAUSA300_RS12570 ← / ← SAUSA300_RS12575	membrane protein/oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,445,965	0	G	A	100.0%	18.0 / NA	8	intergenic (‑474/+13)	SAUSA300_RS12570/SAUSA300_RS12575	membrane protein/oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)

ACACACCTTTACCGACTATTTAAAATACACTTCACCAATTCATTTTAATTTAATGGATTGAAGTAACTAAATTAATATTATGTTGTTCAATTAAAAGCTTCATACAAACCTAATCTATTTGCACTCCACCGCTAACACCGAACACTTGTCCGGTTGTATAACTTGATTCTTCTGACGCTAATAGCACATAAG  >  NC_007793/2445869‑2446060                                                                                                 |                                                                                                acacacCTTTACCGACTATTTAAAATACACTTCACCAATTCATTTTAATTTAATGGATTGAAGTAACTAAATTAATATTATGTTGTTCAATTAAAAACtt                                                                                              >  1:3983905/1‑100 (MQ=255)  cacacCTTTACCGACTATTTAAAATACACTTCACCAATTCATTTTAATTTAATGGATTGAAGTAACTAAATTAATATTATGTTGTTCAATTAAAAACTTc                                                                                             >  1:4405285/1‑100 (MQ=255)                       aaaTACACTTCACCAATTCATTTTAATTTAATGGATTGAAGTAACTAAATTAATATTATGTTGTTCAATTAAAAACTTCATACAAACCTAATCTATTTGc                                                                        <  1:1878075/100‑1 (MQ=255)                        aaTACACTTCACCAATTCATTTTAATTTAATGGATTGAAGTAACTAAATTAATATTATGTTGTTCAATTAAAAACTTCATACAAACCTAATCTATTTGCa                                                                       <  1:1752787/100‑1 (MQ=255)                                  aCCAATTCATTTTAATTTAATGGATTGAAGTAACTAAATTAATATTATGTTGTTCAATTAAAAACTTCATACAAACCTAATCTATTTGCACTCCACCGCt                                                             <  1:3103268/100‑1 (MQ=255)                                   gatcttctctttaatttaaTGGATTGAAGTAACTAAATTAATATTATGTTGTTCAATTAAAAACTTCATACAAACCTAATCTATTTGCACTCCACCGCTa                                                            <  1:5071957/91‑1 (MQ=255)                                                                                       tCAATTAAAAACTTCATACAAACCTAATCTATTTGCACTCCACCGCTAACACCGAACACTTGTCCGGTTGTATAACTTGATTCTTCTGACGCTAATAGca        <  1:3782175/100‑1 (MQ=255)                                                                                             aaaaACTTCATACAAACCTAATCTATTTGCACTCCACCGCTAACACCGAACACTTGTCCGGTTGTATAACTTGATTCTTCTGACGCTAATAGCACATAAg  >  1:2211427/1‑100 (MQ=255)                                                                                                 |                                                                                                ACACACCTTTACCGACTATTTAAAATACACTTCACCAATTCATTTTAATTTAATGGATTGAAGTAACTAAATTAATATTATGTTGTTCAATTAAAAGCTTCATACAAACCTAATCTATTTGCACTCCACCGCTAACACCGAACACTTGTCCGGTTGTATAACTTGATTCTTCTGACGCTAATAGCACATAAG  >  NC_007793/2445869‑2446060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑