Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,444,625	C→A	V25L (GTA→TTA)	SAUSA300_RS12565 ←	sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,444,625	0	C	A	100.0%	55.3 / NA	20	V25L (GTA→TTA)	SAUSA300_RS12565	sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/20);  total (0/20)

TGATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCTGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGCATTGCTCTTTTTATGTACTTCCATCCTTTTTACCTGCTTTCCAATT  >  NC_007793/2444530‑2444719                                                                                                |                                                                                               tGATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCGACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCt                                                                                            <  1:1796798/100‑1 (MQ=255) tGATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCt                                                                                            <  1:143699/100‑1 (MQ=255) tGATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCt                                                                                            <  1:3633231/100‑1 (MQ=255)  gATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTa                                                                                           <  1:4160248/100‑1 (MQ=255)  gATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTa                                                                                           <  1:818702/100‑1 (MQ=255)  gATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTa                                                                                           <  1:1804803/100‑1 (MQ=255)   aTTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTaa                                                                                          <  1:5391971/100‑1 (MQ=255)    ttCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTagc                                                                                         <  1:4409962/100‑3 (MQ=255)               tCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTAAAAACAACGCcac                                                                              <  1:4327452/100‑1 (MQ=255)                cATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTAAAAACAACGCcaca                                                                             <  1:4962129/100‑1 (MQ=255)                 aTTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTAAAAACAACGCCACaa                                                                            <  1:2304303/100‑1 (MQ=255)                             acaATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTAAAAACAACGCCACAAATAATAACAAgg                                                                <  1:828634/100‑1 (MQ=255)                                aTTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTAAAAACAACGCCACAAATAATAACAAGGGGa                                                             <  1:4861627/100‑1 (MQ=255)                                  tACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTAAAAACAACGCCACAAATAATAACAAGGGGaat                                                           <  1:729629/100‑1 (MQ=255)                                             tAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTAAGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGc                                                <  1:1067534/100‑1 (MQ=255)                                                                                  cgtgATAATACCTAAGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGCATTGCTCTTTTTATGTACTTCCATCCTTTTTACCTga           <  1:3760385/99‑2 (MQ=255)                                                                                   gtgATAATACCTAAGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGCATTGCTCTTTTTATGTACTTCCATCCTTTTTACCTGca          <  1:3691746/100‑2 (MQ=255)                                                                                     gATAATACCTAAGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGCATTGCTCTTTTTATGTACTTCCATCCTTTTTACCTGCttt        <  1:4799368/100‑1 (MQ=255)                                                                                       tccgatcTAAGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGCATTGCTCTTTTTATGTACTTCCATCCTTTTTACCTGCtttag      <  1:2775933/94‑3 (MQ=255)                                                                                           aCCTAAGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGCATTGCTCTTTTTATGTACTTCCATCCTTTTTACCTGCTTTCCAAtt  <  1:3253939/100‑1 (MQ=255)                                                                                                |                                                                                               TGATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCTGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGCATTGCTCTTTTTATGTACTTCCATCCTTTTTACCTGCTTTCCAATT  >  NC_007793/2444530‑2444719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑