Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,443,999	C→A	P233P (CCG→CCT)	SAUSA300_RS12565 ←	sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,443,999	0	C	A	100.0%	21.4 / NA	9	P233P (CCG→CCT)	SAUSA300_RS12565	sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/9);  total (0/9)

CTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAAT  >  NC_007793/2443905‑2444079                                                                                               |                                                                                 cTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTAc                                                                             <  1:565355/100‑1 (MQ=255)        aCCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACTTTa                                                                      <  1:3372673/100‑1 (MQ=255)           aaTGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACTTTAAtt                                                                   <  1:3782831/100‑1 (MQ=255)           aaTGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACTTTAAtt                                                                   <  1:4942337/100‑1 (MQ=255)                                                    tGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGcc                          <  1:1193897/100‑1 (MQ=255)                                                    tGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGcc                          <  1:276093/100‑1 (MQ=255)                                                      aCATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATACTTTTTAGCATGATTTAAAGAAATGCCAt                        <  1:3911776/100‑1 (MQ=255)                                                                    taatGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATAc          <  1:293866/100‑1 (MQ=255)                                                                            aTACCAATACTAAGATATAAGGTTCAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGaataat  <  1:2560411/100‑1 (MQ=255)                                                                                               |                                                                                 CTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAAT  >  NC_007793/2443905‑2444079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑