Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,420,692	G→A	A76T (GCG→ACG)	SAUSA300_RS12445 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,420,692	0	G	A	87.5%	11.6 / ‑4.1	8	A76T (GCG→ACG)	SAUSA300_RS12445	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (4/3);  total (4/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

ACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACAATATTCATAGTTCTACAGTGCCTAAGTTATATCACCTCCATCAAAAGTACGCGCTTACATCATTGATACACAAGACATCATGACATAGTTGTATACTGTCTAATCTTCTACTATACTAA  >  NC_007793/2420602‑2420760                                                                                           |                                                                     aCGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACAATATTCATAGTTCTACAGTGCCTAAGTTATATCACCTCCATCAAAAGTACACGCTTAcat                                                             >  1:219697/1‑100 (MQ=255)             ttaattGATCCAGTTTTTCCACATTACAATATTCATAGTTCTACAGTGCCTAAGTTATATCACCTCCATCAAAAGTACACGCTTACATCATTGATAcaca                                                 <  1:2928592/100‑1 (MQ=255)             ttaattGATCCAGTTTTTCCACATTACAATATTCATAGTTCTACAGTGCCTAAGTTATATCACCTCCATCAAAAGTACACGCTTACATCATTGATAcaca                                                 <  1:3704742/100‑1 (MQ=255)                               ccACATTACAATATTCATAGTTCTACAGTGCCTAAGTTATATCACCTCCATCAAAAGTACACGCTTACATCATTGATACACAAGACATCATGACATAGtg                               <  1:449758/100‑2 (MQ=255)                                                     cTACAGTGCCTAAGTTATATCACCTCCATCAAAAGTACACGCTTACATCATTGATACACAAGACATCATGACATAGTTGTATACTGTCTAATCTTctact         >  1:1876172/1‑100 (MQ=255)                                                       aCAGTGCCTAAGTTATATCACCTCCATCAAAAGTACACGCTTACATCATTGATACACAAGACATCATGACATAGTTGTATACTGTCTAATCTTCtactat       >  1:1524492/1‑100 (MQ=255)                                                       aCAGTGCCTAAGTTATATCACCTCCATCAAAAGTACACGCTTACATCATTGATACACAAGACATCATGACATAGTTGTATACTGTCTAATCTTCtactat       >  1:3128015/1‑100 (MQ=255)                                                            gCCTAAGTTATATCGCCTCCATCAAAAGTACGCGCTTACATCATTGATACACAAGACATCATGACATAGTTGTATACTGTCTAATCTTCTACTATACTaa  <  1:1381659/100‑1 (MQ=255)                                                                                           |                                                                     ACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACAATATTCATAGTTCTACAGTGCCTAAGTTATATCACCTCCATCAAAAGTACGCGCTTACATCATTGATACACAAGACATCATGACATAGTTGTATACTGTCTAATCTTCTACTATACTAA  >  NC_007793/2420602‑2420760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑