Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,414,505	A→G	L29L (TTA→CTA)	SAUSA300_RS12410 ←	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,414,505	0	A	G	100.0%	67.4 / NA	26	L29L (TTA→CTA)	SAUSA300_RS12410	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/25);  total (1/25)

TCAATTTGATACAAATCATTCAACATAATAATGGTGACATAATTTCTTTTTAGATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAACCAATATATAATTTTTAAGTTCGCTTCCTTCACACTACTCATTTTCATACTTTTATAAATATGTAGTGAATAATTATCTG  >  NC_007793/2414408‑2414585                                                                                                  |                                                                                 tCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGcc                                                                                <  1:1069136/100‑1 (MQ=255) tCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGcc                                                                                <  1:5374188/100‑1 (MQ=255) tCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGcc                                                                                <  1:4740553/100‑1 (MQ=255) tCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGcc                                                                                <  1:2328759/100‑1 (MQ=255) tCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGcc                                                                                <  1:5736152/100‑1 (MQ=255)  taaTTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                               <  1:3376842/99‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGcct                                                                               <  1:5538488/100‑2 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                               <  1:5351802/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                               <  1:511965/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                               <  1:4029809/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                               <  1:3523300/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                               <  1:3390032/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                               <  1:3084664/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                               <  1:1947719/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                               <  1:154346/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                               <  1:139375/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGGAGCCa                                                                               <  1:2714415/100‑1 (MQ=255)     tttGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCaaga                                                                            <  1:5777027/100‑3 (MQ=255)     tttGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCActa                                                                            <  1:4264881/100‑1 (MQ=255)                tCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTa                                                                 <  1:937957/100‑1 (MQ=255)                 cATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTaa                                                                <  1:1180675/100‑1 (MQ=255)                     cAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTCTCAAGATc                                                            >  1:1653846/1‑100 (MQ=255)                         ataataATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGctt                                                        <  1:1718382/100‑1 (MQ=255)                             taatGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGcttcctg                                                    <  1:3473139/100‑2 (MQ=255)                                  gTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACGCCTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCacac                                               <  1:2690505/100‑1 (MQ=255)                                                                               ttccgatcTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCACACTACTCATTTTCATACTTTTATAAATATGTAGTGAATAATTATCTg  <  1:5260714/93‑1 (MQ=255)                                                                                                  |                                                                                 TCAATTTGATACAAATCATTCAACATAATAATGGTGACATAATTTCTTTTTAGATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAACCAATATATAATTTTTAAGTTCGCTTCCTTCACACTACTCATTTTCATACTTTTATAAATATGTAGTGAATAATTATCTG  >  NC_007793/2414408‑2414585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑