Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,388,654	C→T	intergenic (‑87/‑39)	SAUSA300_RS12240 ← / → SAUSA300_RS12245	transcriptional regulator/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,388,654	0	C	T	100.0%	115.6 / NA	42	intergenic (‑87/‑39)	SAUSA300_RS12240/SAUSA300_RS12245	transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/42);  total (0/42)

TTACTCATAATATGATGCCTCCTATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTGGACAACTTGTTG  >  NC_007793/2388560‑2388726                                                                                               |                                                                         ttaCTCATAATATGATGCCTCCTATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTAtttt                                                                     <  1:5629852/100‑1 (MQ=255)            atgatgCCTCCTATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACat                                                          <  1:4124146/100‑2 (MQ=255)                          cgatcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTg                                            <  1:2159710/97‑1 (MQ=255)                           tttCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTgg                                           <  1:4722568/100‑1 (MQ=255)                             tcttctTGGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:1631557/100‑1 (MQ=255)                             tcttctTATATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:946592/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAGTTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:5115849/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAGTTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:1033092/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGTTGGCt                                         <  1:4059461/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGca                                         <  1:4102363/100‑2 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:3860324/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:984089/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:5151152/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:4690914/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:4075018/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:3214410/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:3059487/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:178060/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:1212141/100‑1 (MQ=255)                             tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTAGCTATTACAAATATTAAATTGCTGGCt                                         <  1:3960918/100‑1 (MQ=255)                             tcttctTAGATGTATGTAATTCATATTATTATACAATTACTTAAATATATTATATATCAAACTTAGTATTTTATCTATTGCAAATATTAAATTGCTGGCt                                         <  1:5188843/100‑1 (MQ=255)                             tcttctCAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                         <  1:2762802/100‑1 (MQ=255)                              cttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCCATTACAAATATTAAATTGCTGGCta                                        <  1:4037532/100‑1 (MQ=255)                               ttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCtat                                       <  1:2613928/100‑1 (MQ=255)                               ttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCtat                                       <  1:1633994/100‑1 (MQ=255)                               ttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCtat                                       <  1:899826/100‑1 (MQ=255)                               ttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCtat                                       <  1:3585904/100‑1 (MQ=255)                               ttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCtat                                       <  1:3265822/100‑1 (MQ=255)                                tcttCGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCtata                                      <  1:1318722/100‑1 (MQ=255)                                tcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGcgcac                                      <  1:3683561/100‑5 (MQ=255)                                tcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCtatt                                      <  1:4268932/100‑2 (MQ=255)                                tcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCtata                                      <  1:5349856/100‑1 (MQ=255)                                    aGATGTTTGTAATTCATATTATTATACAATTCCTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCtt                                  <  1:1420099/100‑1 (MQ=255)                                    aGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCtt                                  <  1:430567/100‑1 (MQ=255)                                      aTGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGt                                <  1:3178709/100‑1 (MQ=255)                                       tGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGtt                               <  1:5550175/100‑1 (MQ=255)                                        gTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGttt                              <  1:2414042/100‑1 (MQ=255)                                        gTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGttt                              <  1:2562991/100‑1 (MQ=255)                                        gTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGttt                              <  1:2695450/100‑1 (MQ=255)                                                         ttatACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTgg             <  1:1072801/100‑1 (MQ=255)                                                           ataCAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTGGAc           <  1:5454736/100‑1 (MQ=255)                                                                    cTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTGGACAACttgttg  <  1:1534758/100‑1 (MQ=255)                                                                                               |                                                                         TTACTCATAATATGATGCCTCCTATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTGGACAACTTGTTG  >  NC_007793/2388560‑2388726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑