Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,381,180	A→C	T784T (ACT→ACG)	SAUSA300_RS12210 ←	multidrug transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,381,180	0	A	C	100.0%	216.1 / NA	81	T784T (ACT→ACG)	SAUSA300_RS12210	multidrug transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/75);  total (6/75)

ATTTAATCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACAGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGCAGAAATACCATTT  >  NC_007793/2381083‑2381270                                                                                                  |                                                                                           aTTTAATCGTGCCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGTCTTTAACATCAACCGt                                                                                          <  1:757554/100‑1 (MQ=255)  tttAATCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGtt                                                                                         <  1:2723550/100‑1 (MQ=255)  tttAATCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGtt                                                                                         <  1:4350562/100‑1 (MQ=255)   ttAATCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGttt                                                                                        <  1:5480472/100‑1 (MQ=255)    tAATCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGtttt                                                                                       <  1:1772557/100‑1 (MQ=255)     aaTCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTa                                                                                      >  1:650457/1‑100 (MQ=255)       tCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTAcc                                                                                    <  1:505750/100‑1 (MQ=255)       tCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTAcc                                                                                    <  1:3783023/100‑1 (MQ=255)       tCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTAcc                                                                                    <  1:1302055/100‑1 (MQ=255)       tCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTAcc                                                                                    <  1:3144816/100‑1 (MQ=255)       tCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTAcc                                                                                    <  1:4703116/100‑1 (MQ=255)        cGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCa                                                                                   <  1:4689617/100‑1 (MQ=255)         gTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCac                                                                                  <  1:1038193/100‑2 (MQ=255)         gTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTCTTACCag                                                                                  >  1:5548037/1‑99 (MQ=255)           accaccAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTAccctag                                                                                <  1:1862964/100‑5 (MQ=255)            ccaccaGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCAtttt                                                                               >  1:1323149/1‑100 (MQ=255)               ccaGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCtt                                                                            <  1:5658731/100‑1 (MQ=255)               ccaGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCtt                                                                            <  1:1656338/100‑1 (MQ=255)                    cGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACa                                                                       >  1:2824242/1‑100 (MQ=255)                        tttttCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCATTAACAGTca                                                                   <  1:624854/100‑2 (MQ=255)                        tttttCAAAGTTATATTATTCAACTTATCTTATGGCGAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGtgg                                                                   <  1:468154/100‑3 (MQ=255)                         ttttCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGt                                                                  <  1:380009/100‑1 (MQ=255)                         ttttCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGt                                                                  <  1:5253457/100‑1 (MQ=255)                         ttttCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGt                                                                  <  1:5604813/100‑1 (MQ=255)                          tttCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTa                                                                 <  1:858578/100‑1 (MQ=255)                          tttCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTa                                                                 <  1:4753292/100‑1 (MQ=255)                          tttCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTa                                                                 <  1:3282379/100‑1 (MQ=255)                          tttCAAAGTAATATTATTCAAATTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTa                                                                 <  1:5520943/100‑1 (MQ=255)                           ttCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCATCCGTTTTACCATTTTCTTTAACAGTCGTat                                                                <  1:4039252/100‑2 (MQ=255)                             cAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACt                                                              <  1:4477163/100‑1 (MQ=255)                                        tattCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTgg                                                   <  1:2617544/100‑1 (MQ=255)                                          ttCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTa                                                 <  1:5202840/100‑1 (MQ=255)                                          ttCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTa                                                 <  1:2237050/100‑1 (MQ=255)                                          ttCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGGTTGACATTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGGTTTTTCTGGTa                                                 <  1:5028841/100‑1 (MQ=255)                                          gacgtgtgctcttccGATCTGGCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTa                                                 <  1:590341/91‑1 (MQ=255)                                                 tATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAgggcaa                                          <  1:3783800/100‑6 (MQ=255)                                                      ttccgatcttCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTttcattca                                     <  1:3832478/91‑1 (MQ=255)                                                      tctGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTttcattca                                     <  1:3229497/100‑1 (MQ=255)                                                      tctGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTttcattca                                     <  1:2871975/100‑1 (MQ=255)                                                      tccgatctgTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTttcattca                                     <  1:921215/92‑1 (MQ=255)                                                      tccgatctgTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTttcattca                                     <  1:3489315/92‑1 (MQ=255)                                                         gACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCGACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGt                                  >  1:3900054/1‑100 (MQ=255)                                                         gACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGt                                  >  1:3332821/1‑100 (MQ=255)                                                         gACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATCCAAGt                                  <  1:5616138/100‑1 (MQ=255)                                                           ccAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGc                                <  1:4889391/100‑1 (MQ=255)                                                             gatCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTggga                              <  1:3147133/98‑4 (MQ=255)                                                             gatCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAt                              <  1:700952/98‑1 (MQ=255)                                                             aGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTATCTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAt                              <  1:2261952/100‑1 (MQ=255)                                                              gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                             <  1:5207450/100‑1 (MQ=255)                                                              gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                             <  1:1295199/100‑1 (MQ=255)                                                              gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                             <  1:4036727/100‑1 (MQ=255)                                                              gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                             <  1:1989696/100‑1 (MQ=255)                                                              gTCTGTATGCTTATTTTGTTTGACTTTAACATCATCCGTTTTACCATTTTCTTGAACAGTCGTAACTGTTGTTTCTGGTAAGTTTTCATTCAAGTGCAta                             <  1:2423182/100‑2 (MQ=255)                                                               tCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTg                            <  1:1591810/100‑1 (MQ=255)                                                                cTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGc                           <  1:3540817/100‑1 (MQ=255)                                                                cTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGc                           <  1:5607510/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTTGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:1906544/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:2502114/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:3678958/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:2265031/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:1917080/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:914814/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:3721939/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:2724222/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:2859740/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:3033995/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:3836327/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                          <  1:3499197/100‑1 (MQ=255)                                                                 tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTGTCATTCAAGTGCATTGCa                          <  1:4628465/100‑1 (MQ=255)                                                                  gTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTgagc                         <  1:602198/100‑4 (MQ=255)                                                                  gTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCaa                         <  1:5168524/100‑1 (MQ=255)                                                                            tttgtttgACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGc               <  1:5402462/100‑1 (MQ=255)                                                                            tttgtttgACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGc               <  1:3617613/100‑1 (MQ=255)                                                                            tttgtttgACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGc               <  1:704379/100‑1 (MQ=255)                                                                            tttgtttgACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGc               <  1:702535/100‑1 (MQ=255)                                                                            tttgtttgACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGc               <  1:3296481/100‑1 (MQ=255)                                                                            tttgtttgACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGGCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGc               <  1:5423840/100‑1 (MQ=255)                                                                             ttgtttgACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTgag              <  1:1982784/100‑3 (MQ=255)                                                                              tgtttgACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGCAg             <  1:4671927/100‑1 (MQ=255)                                                                                       ttAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGCAGAAATACCAt    <  1:3141543/100‑1 (MQ=255)                                                                                         aaCATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGCAGAAATACCAtta  <  1:108663/100‑2 (MQ=255)                                                                                                  |                                                                                           ATTTAATCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACAGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGCAGAAATACCATTT  >  NC_007793/2381083‑2381270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑