Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,376,290	C→G	G77G (GGC→GGG)	SAUSA300_RS12180 →	GNAT family acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,376,290	0	C	G	100.0%	84.3 / NA	32	G77G (GGC→GGG)	SAUSA300_RS12180	GNAT family acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (27/5);  total (27/5)

TTGAATCACCTGGTGTATTTTCGATTAAAGAAAACGATTCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGCCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCTTTAAC  >  NC_007793/2376193‑2376386                                                                                                  |                                                                                                 ttGAATCACCTGGTGTATTTTCGATTAAAGAAAACGATTCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATGGGgcc                                                                                                <  1:3160374/100‑1 (MQ=255)     ttCACCTGGTGTATTTTCGATTAAAGAAAACGATTCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTc                                                                                            >  1:1657744/2‑100 (MQ=255)      gcACCTGGTGTATTTTCGATTAAAGAAAACGATTCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCg                                                                                           >  1:274601/2‑100 (MQ=255)           tGGTGTATTTTCGATTAAAGAAAACGATTCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCt                                                                                      >  1:2286857/1‑100 (MQ=255)            ggTGTATTTTCGATTAAAGAAAACGATTCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTg                                                                                     <  1:5788328/100‑1 (MQ=255)            ggTGTATTTTCGATTAAAGAAAACGATTCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTg                                                                                     <  1:4774054/100‑1 (MQ=255)            ggTGTATTTTCGATTAAAGAAAACGATTCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTg                                                                                     >  1:2633689/1‑100 (MQ=255)             gtgtATTTTCGATTAAAGCAAACGATTCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTGa                                                                                    <  1:1119909/100‑1 (MQ=255)                   aaacGATTAAAGAAAACGATTCGATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTGACAATTa                                                                              >  1:4305726/4‑100 (MQ=255)                                      ttCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACg                                                           >  1:4724265/1‑100 (MQ=255)                                         aatcaaAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGttt                                                        >  1:3706547/1‑100 (MQ=255)                                              aaGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAAc                                                   >  1:4989439/1‑100 (MQ=255)                                                               tttGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGa                                  >  1:493415/1‑100 (MQ=255)                                                               tttGCATACGATAAAAATAAATTCAAAGTCATAGGGACTTTCGTGGCTGACAATTATGTAATATCTGTCGATAAGTTTGAAACGCTATTTAAAGCAATGa                                  >  1:674715/1‑100 (MQ=255)                                                               cttGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGa                                  >  1:3272298/2‑100 (MQ=255)                                                                 tGCATACGATAAAAATAAATTCAAAGTCATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACt                                >  1:4944457/1‑100 (MQ=255)                                                                               ataaatTCAAAGTCATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGa                  <  1:2591540/100‑1 (MQ=255)                                                                                   atTCAAAGTCATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAACGACTTCGAACCAACCTGACGAt              >  1:2709096/1‑100 (MQ=255)                                                                                        aaGTCATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCga         <  1:2891260/100‑2 (MQ=255)                                                                                           tCATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCtt      >  1:101609/1‑100 (MQ=255)                                                                                            cATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCttt     >  1:3328937/1‑100 (MQ=255)                                                                                            cATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCttt     >  1:1158858/1‑100 (MQ=255)                                                                                            cATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCttt     >  1:5049301/1‑100 (MQ=255)                                                                                            cATAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCttt     >  1:2423064/1‑100 (MQ=255)                                                                                             caaGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCTTTa    >  1:1173635/3‑100 (MQ=255)                                                                                             agaGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCTTTa    >  1:5451251/3‑100 (MQ=255)                                                                                             aTAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCTTTa    >  1:3160243/1‑100 (MQ=255)                                                                                             aTAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCTTTa    >  1:4363619/1‑100 (MQ=255)                                                                                             aTAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCTTTa    >  1:3846148/1‑100 (MQ=255)                                                                                              tAGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCTTTaa   >  1:4910476/1‑100 (MQ=255)                                                                                              caGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCTTTaa   >  1:835892/2‑100 (MQ=255)                                                                                               aGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGGCGTCTTTAAc  >  1:3962499/1‑100 (MQ=255)                                                                                               aGGGCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCTTTAAc  >  1:4138955/1‑100 (MQ=255)                                                                                                  |                                                                                                 TTGAATCACCTGGTGTATTTTCGATTAAAGAAAACGATTCAATCAAAGCAATCATATTGTCTTTTGCATACGATAAAAATAAATTCAAAGTCATAGGCCCTTTCGTGGCTGACAATTATGTATTATCTGTCGATACGTTTGAAACGCTATTTAAAGCAATGACTTCGAACCAACCTGACGATGCCGTCTTTAAC  >  NC_007793/2376193‑2376386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑