Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,344,945	C→T	E333E (GAG→GAA)	SAUSA300_RS11940 ←	acetolactate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,344,945	0	C	T	100.0%	60.4 / NA	23	E333E (GAG→GAA)	SAUSA300_RS11940	acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/23);  total (0/23)

GAATTCCTTCTTCATGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGCTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGCATATAATTAGTAATTTCAGCTTGAA  >  NC_007793/2344848‑2345039                                                                                                  |                                                                                               gAATTCCTTCTTCATGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTc                                                                                              <  1:1855456/100‑1 (MQ=255)     tccgatctcATGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTCATcc                                                                                          <  1:1071283/93‑1 (MQ=255)       gatcttcATGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCAc                                                                                        <  1:5603429/98‑1 (MQ=255)         tcttcATGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACtt                                                                                      <  1:583807/100‑1 (MQ=255)         ccgatctGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACta                                                                                      <  1:1325079/94‑2 (MQ=255)             tctGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTc                                                                                  <  1:4701058/98‑1 (MQ=255)             cATGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTc                                                                                  <  1:2560925/100‑1 (MQ=255)             cATGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTc                                                                                  <  1:585270/100‑1 (MQ=255)             cATGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTc                                                                                  <  1:2605504/100‑1 (MQ=255)               tGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAGATGTTGTTGATTTATAAATGGTTCATCCACTTTTTCAg                                                                                <  1:1818153/100‑1 (MQ=255)                                              tatGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTAcc                                                 <  1:1718820/100‑1 (MQ=255)                                               atGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCa                                                <  1:1715902/100‑1 (MQ=255)                                                                    ttCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGc                           <  1:2411413/100‑1 (MQ=255)                                                                    ttCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGc                           <  1:2593997/100‑1 (MQ=255)                                                                    ttCGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGc                           <  1:3357641/100‑1 (MQ=255)                                                                      cGTCTAAATGTTGTTGATTTATAAATGGTTCATCCTCTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTGCCAATCAACTCTTTTTTCGGTTGCat                         <  1:1787169/100‑1 (MQ=255)                                                                      cGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGCat                         <  1:866864/100‑1 (MQ=255)                                                                      cGTCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAAACGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGCat                         <  1:1723397/100‑1 (MQ=255)                                                                       gTCTAAATGTTGTCGATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGCata                        <  1:5200917/100‑1 (MQ=255)                                                                       atCTAAATGTTGTTGATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGCata                        <  1:1989111/99‑1 (MQ=255)                                                                                    tgATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGCATATAATTAGTAAttt           <  1:541345/100‑1 (MQ=255)                                                                                     gATTTATAAATGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGCATATAATTAGTAATTTc          <  1:2284953/100‑1 (MQ=255)                                                                                             aaTGGTTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGCATATAATTAGTAATTTCAGCTTGag  <  1:5473343/100‑2 (MQ=255)                                                                                                  |                                                                                               GAATTCCTTCTTCATGCGTCGCTTTAATACCAGTTTCTTCATCAATATGTGTTCTTAATTGTTCTAATTCGTCTAAATGTTGTTGATTTATAAATGGCTCATCCACTTTTTCAGAAATCATTTCAATCGTTTTAGCAATATTACCAATCAACTCTTTTTTCGGTTGCATATAATTAGTAATTTCAGCTTGAA  >  NC_007793/2344848‑2345039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑