Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 2,300,166 A→C L174V (TTA→GTA)  SAUSA300_RS11710 ← multidrug MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,300,1660AC97.5% 110.4 / ‑4.1 40L174V (TTA→GTA) SAUSA300_RS11710multidrug MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (2/37);  total (2/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

AATTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTAAAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAGCTGTAGGTCCTATTGCTGGTGCAA  >  NC_007793/2300090‑2300243
                                                                            |                                                                             
aaTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGcc                                                        <  1:714376/100‑1 (MQ=255)
 aTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCa                                                       <  1:3395285/100‑1 (MQ=255)
 aTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCa                                                       <  1:973730/100‑1 (MQ=255)
 aTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCa                                                       <  1:4164386/100‑1 (MQ=255)
 aTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCa                                                       <  1:2079005/100‑1 (MQ=255)
 aTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCa                                                       <  1:1304733/100‑1 (MQ=255)
  tttCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCaa                                                      <  1:520305/100‑1 (MQ=255)
    tCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAAcg                                                    <  1:4013269/100‑2 (MQ=255)
    tCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACt                                                    <  1:1773997/100‑1 (MQ=255)
    tCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACt                                                    <  1:5431481/100‑1 (MQ=255)
            ttCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGAt                                            <  1:5673007/100‑1 (MQ=255)
             tCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATc                                           <  1:3544676/100‑1 (MQ=255)
             tCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATc                                           <  1:5085793/100‑1 (MQ=255)
             tCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATc                                           <  1:5775572/100‑1 (MQ=255)
             tCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATc                                           <  1:1390337/100‑1 (MQ=255)
             tCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATc                                           <  1:345171/100‑1 (MQ=255)
             tCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATc                                           <  1:2619236/100‑1 (MQ=255)
             tCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATc                                           <  1:2782382/100‑1 (MQ=255)
                         aaCTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATcc                               >  1:3464096/1‑100 (MQ=255)
                            tCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgt                            <  1:2339294/100‑1 (MQ=255)
                            ccGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgt                            >  1:3077696/2‑100 (MQ=255)
                                  ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                      <  1:3145721/100‑1 (MQ=255)
                                  ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                      <  1:906522/100‑1 (MQ=255)
                                  ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                      <  1:3077344/100‑1 (MQ=255)
                                  ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                      <  1:4657312/100‑1 (MQ=255)
                                  ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATAGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                      <  1:1623959/100‑1 (MQ=255)
                                  ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTAAAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtagctgt                      <  1:2690876/100‑1 (MQ=255)
                                  ttGAAACCAAACCGAAAACAAAGGCAAATATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                      <  1:461168/100‑1 (MQ=255)
                                         caaaccGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCt               <  1:4149385/100‑1 (MQ=255)
                                         caaaccGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCt               <  1:2067351/100‑1 (MQ=255)
                                         caaaccGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCt               <  1:4418903/100‑1 (MQ=255)
                                         caaaccGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCt               <  1:4251834/100‑1 (MQ=255)
                                         caaaccGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCt               <  1:2889770/100‑1 (MQ=255)
                                            accGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTAtt            <  1:1003209/100‑1 (MQ=255)
                                                aaaaCAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTg        <  1:2408234/100‑1 (MQ=255)
                                                    cAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAGCTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGc    <  1:1665723/100‑1 (MQ=255)
                                                    cAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTgg    <  1:3363715/100‑2 (MQ=255)
                                                    cAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGc    <  1:14564/100‑1 (MQ=255)
                                                     aaaGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGACCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCa   <  1:3968343/100‑1 (MQ=255)
                                                      aaGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCaa  <  1:254844/100‑1 (MQ=255)
                                                                            |                                                                             
AATTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTAAAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAGCTGTAGGTCCTATTGCTGGTGCAA  >  NC_007793/2300090‑2300243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑