Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,291,661	G→A	intergenic (+41/+187)	SAUSA300_RS11650 → / ← SAUSA300_RS11655	transposase/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,291,661	0	G	A	100.0%	14.4 / NA	10	intergenic (+41/+187)	SAUSA300_RS11650/SAUSA300_RS11655	transposase/tRNA‑Lys
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/3);  total (7/3)

AATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCA  >  NC_007793/2291570‑2291717                                                                                            |                                                         aataatGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACATATAatga                                                  <  1:4569797/100‑1 (MQ=17)      aggTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACATATAATGATGAtt                                             >  1:5480125/2‑100 (MQ=17)       ggTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACATATAATGATGAttt                                            >  1:4318025/1‑100 (MQ=17)        atGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACATATAATGATGAtttt                                           >  1:2040352/2‑100 (MQ=17)           cTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACATATAATGATGATTTTCAg                                        >  1:2381670/1‑100 (MQ=17)             ataGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACATATAATGATGATTTTCAGCt                                      >  1:2342774/1‑100 (MQ=17)             ataGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACATATAATGATGATTTCCAGCt                                      >  1:5750587/1‑100 (MQ=12)             ataGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGATACAGAAATTTCAACAACTTTAAAGCACATATAATGATGATTTTCAGCt                                      >  1:3761739/1‑100 (MQ=12)                       ttAATAATAAGATAAGATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACATATAATGATGATTTTCAGCTTGTATAAAgg                            <  1:3746701/100‑1 (MQ=255)                                                 tGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACATATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAGAAGAAGACAACCAAGCCCa  <  1:3921374/100‑1 (MQ=2)                                                                                            |                                                         AATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCA  >  NC_007793/2291570‑2291717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑