Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,276,564	A→G	E56G (GAA→GGA)	SAUSA300_RS11615 →	PTS mannitol transporter subunit IIA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,276,564	0	A	G	100.0%	56.4 / NA	22	E56G (GAA→GGA)	SAUSA300_RS11615	PTS mannitol transporter subunit IIA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/0);  total (22/0)

AGCAGGTAAAGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGAAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGTTGACTG  >  NC_007793/2276472‑2276660                                                                                             |                                                                                                 aGCAGGTAAAGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGc                                                                                           >  1:3326289/1‑100 (MQ=255)  acAGGTAAAGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGCt                                                                                          >  1:2984159/2‑100 (MQ=255)        taaGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCaa                                                                                    >  1:2040704/2‑100 (MQ=255)        taaGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCaa                                                                                    >  1:4830717/2‑100 (MQ=255)         caGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCAAt                                                                                   >  1:131541/2‑100 (MQ=255)                   tGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGc                                                                         >  1:5438022/1‑100 (MQ=255)                   tGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGCGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGc                                                                         >  1:4607283/1‑100 (MQ=255)                          ggtgCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGAtc                                                                  >  1:2920510/1‑99 (MQ=255)                                                 gcAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCAc                                           >  1:1563146/2‑100 (MQ=255)                                                      cAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAgg                                      >  1:2747820/1‑100 (MQ=255)                                                                   tGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACAttatta                         >  1:4524136/1‑100 (MQ=255)                                                                        caGTCGTATCAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAAt                    >  1:3023181/2‑100 (MQ=255)                                                                         aGTCGTATCAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATc                   >  1:43079/1‑100 (MQ=255)                                                                                acAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAg            >  1:109685/2‑100 (MQ=255)                                                                                 cAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAgg           >  1:5578711/1‑100 (MQ=255)                                                                                 cAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAgg           >  1:2730838/1‑100 (MQ=255)                                                                                 cAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAgg           >  1:2212013/1‑100 (MQ=255)                                                                                     gcttATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGtt       >  1:3573872/3‑100 (MQ=255)                                                                                       ttATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGTTGa     >  1:4611794/1‑100 (MQ=255)                                                                                        tATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGTTGAc    >  1:4397159/1‑100 (MQ=255)                                                                                         aTGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGTTGACt   >  1:3807959/1‑100 (MQ=255)                                                                                         aTGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCT‑AAACAAATGTGATTTAATCAGGGTTAACATTATTACAAATCCCTGAAGGCGTTGACTg  >  1:4334917/1‑100 (MQ=255)                                                                                             |                                                                                                 AGCAGGTAAAGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGAAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGTTGACTG  >  NC_007793/2276472‑2276660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑