Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,275,549	T→G	D432E (GAT→GAG)	SAUSA300_RS11610 →	PTS lactose transporter subunit IIB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,275,549	0	T	G	100.0%	90.8 / NA	33	D432E (GAT→GAG)	SAUSA300_RS11610	PTS lactose transporter subunit IIB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/2);  total (31/2)

AAAAACCAAGGTAATCGATTTTTAAACATATTAGTCGTTTGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGATCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTCACTAGATTTAAGTCAATATGATGGCATTATTTCTACTGTG  >  NC_007793/2275463‑2275645                                                                                       |                                                                                                 aaaaaCCAAGGTAATCGATTTTTAAACATATTAGTCGTTTGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTg                                                                                     <  1:2024149/100‑1 (MQ=255)   aaaCCAAGGTAATCGATTTTTAAACATATTAGTCGTTTGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTgag                                                                                   >  1:3016733/1‑100 (MQ=255)      ccAAGGTAATCGATTTTTAAACATATTAGTCGTTTGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCATCTCGTCTATAGCAAGTTTTTAGTaagctc                                                                                >  1:1688973/1‑97 (MQ=255)                      ttAAACATATTAGTCGTTTGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAg                                                                >  1:653083/1‑100 (MQ=255)                          aCATATTAGTCATTTGCAGCAGTGGTATGCGAACTAGTCGTCTATTATCATCTCTTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACTCAAGCAtc                                                            <  1:4354446/100‑1 (MQ=255)                                       ttGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCAtcagtcagatcggaa                                               >  1:4739758/1‑93 (MQ=255)                                       ttGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGcagatcg                                               >  1:5673391/1‑94 (MQ=255)                                       ttGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCgcagat                                               >  1:2329540/1‑95 (MQ=255)                                       ttGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTg                                               >  1:1975146/1‑100 (MQ=255)                                       ttGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGACAGcagatcg                                               >  1:3155264/1‑94 (MQ=255)                                        tGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGa                                              >  1:2730749/1‑100 (MQ=255)                                        tGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGa                                              >  1:943855/1‑100 (MQ=255)                                        tGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGa                                              >  1:941596/1‑100 (MQ=255)                                        tGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGAGAtcgga                                              >  1:3297701/1‑96 (MQ=255)                                        tGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGACAGcagatcgg                                              >  1:380985/1‑93 (MQ=255)                                        agcagcaGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGa                                              >  1:2224316/2‑100 (MQ=255)                                        agcagcaGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGa                                              >  1:1685197/2‑100 (MQ=255)                                         gcagcaGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCAtcaatcagatcggaaga                                             >  1:1781207/1‑91 (MQ=255)                                         gcagcaGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGaa                                             >  1:4426524/1‑100 (MQ=255)                                         gcagcaGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGaa                                             >  1:4540938/1‑100 (MQ=255)                                         gcagcaGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGaa                                             >  1:541749/1‑100 (MQ=255)                                         ctagcagTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGccagatcgg                                             >  1:1562511/3‑92 (MQ=255)                                         acagcagTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGaa                                             >  1:5321022/2‑100 (MQ=255)                                          cagcagTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGcagatcggaa                                            >  1:1841114/1‑91 (MQ=255)                                            gcagTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTc                                          >  1:1168912/1‑100 (MQ=255)                                            gcagTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGGCAGAGATCGGAAgag                                          >  1:685029/1‑98 (MQ=255)                                             tagTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTCa                                         >  1:4092109/2‑100 (MQ=255)                                              tgTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTCAc                                        >  1:4392010/2‑100 (MQ=255)                                              cgTGGTATGGGAACTAGTCGTCTATTTTCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTCAc                                        >  1:3874667/2‑100 (MQ=255)                                               ctGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTCACt                                       >  1:5667446/2‑100 (MQ=255)                                                 ggTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTCACTAg                                     >  1:2926097/1‑100 (MQ=255)                                                    aTGGGAACTAGTCGTCTATTATCAACTCGTCTAAAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTCACTAGAtt                                  >  1:4321328/1‑100 (MQ=255)                                                      gggAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTCACTAGATTTa                                >  1:5118616/1‑100 (MQ=255)                                                                                    agagCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTCACTAGATTTAAGTCAATATGATGGCATTATTTCTACtgtg  >  1:1359911/1‑100 (MQ=255)                                                                                       |                                                                                                 AAAAACCAAGGTAATCGATTTTTAAACATATTAGTCGTTTGCAGCAGTGGTATGGGAACTAGTCGTCTATTATCAACTCGTCTAGATCAAGTTTTTAGTGAGATTGAGCGTATTACACAAGCATCAGTCAGCGATTTGAAGTCACTAGATTTAAGTCAATATGATGGCATTATTTCTACTGTG  >  NC_007793/2275463‑2275645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑