Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,261,786	A→G	L49L (CTT→CTC)	SAUSA300_RS11550 ←	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,261,786	0	A	G	100.0%	134.4 / NA	51	L49L (CTT→CTC)	SAUSA300_RS11550	oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/48);  total (3/48)

TTCCCTCCAGAACCTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACAAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTAAATGGTACATTTTCTTTTGCTAATTGTTGC  >  NC_007793/2261697‑2261882                                                                                          |                                                                                                 gatcttCCAGGACCTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCtt                                                                                        <  1:321030/95‑1 (MQ=255)    ccTCCAGAACCTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCa                                                                                     <  1:2170513/100‑1 (MQ=255)       ccgatctttACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATat                                                                                  <  1:2923101/92‑2 (MQ=255)        cAGAACCTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATAcc                                                                                 <  1:4684137/100‑1 (MQ=255)         tctgccTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCt                                                                                <  1:1693031/96‑1 (MQ=255)          ttaCCTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCtt                                                                               >  1:5598509/3‑100 (MQ=255)          gAACCTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATCTTCAACATCAACGAGAATTGCTTTCATACCtt                                                                               <  1:3943771/100‑1 (MQ=255)          gAACCTACTGAAAAGATAACTTTATCAAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCtt                                                                               <  1:4567062/100‑1 (MQ=255)           aaCCTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTtg                                                                              <  1:2561159/100‑1 (MQ=255)            aCCTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTtgg                                                                             <  1:3172285/100‑2 (MQ=255)             tcTACTGTTAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATAACTtgtg                                                                            <  1:1030002/99‑1 (MQ=255)             tcTACCGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTtgtg                                                                            <  1:3322179/99‑1 (MQ=255)             ccTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTtgtg                                                                            <  1:173566/100‑1 (MQ=255)             ccTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTtgtg                                                                            <  1:2818462/100‑1 (MQ=255)                aCTGAAAAGATAACCTTATCGAATAGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGAtt                                                                         >  1:2818997/1‑100 (MQ=255)                          tAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCAtt                                                               <  1:4789678/100‑1 (MQ=255)                                   agAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATc                                                      <  1:3897027/99‑1 (MQ=255)                                            tAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCt                                             <  1:4931329/100‑1 (MQ=255)                                            tAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCt                                             <  1:1161472/100‑1 (MQ=255)                                            tAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCt                                             <  1:1194248/100‑1 (MQ=255)                                            tAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCt                                             <  1:3551698/100‑1 (MQ=255)                                            tAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCt                                             <  1:3488271/100‑1 (MQ=255)                                            tAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCt                                             <  1:2032653/100‑1 (MQ=255)                                             aaaCGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTa                                            <  1:5786321/100‑1 (MQ=255)                                             aaaCGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTa                                            <  1:1013871/100‑1 (MQ=255)                                             aaaCGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTa                                            <  1:1687636/100‑1 (MQ=255)                                              aaCGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTaa                                           <  1:3880519/100‑1 (MQ=255)                                               aCGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTaag                                          <  1:5081706/100‑2 (MQ=255)                                                  tctcAGTTAAAGTCTCTATTGAATCATTTTCAACATCGACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACAcc                                       <  1:1128925/100‑1 (MQ=255)                                                  tctcAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACAcc                                       <  1:126915/100‑1 (MQ=255)                                                    tcAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcag                                     <  1:3632202/100‑1 (MQ=255)                                                    tcAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcag                                     <  1:3947025/100‑1 (MQ=255)                                                    tcAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTCCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcag                                     <  1:173313/100‑1 (MQ=255)                                                     cAGTTAAAGTCTCTATTGAATCATTTTCAGCATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcagg                                    <  1:4677638/100‑2 (MQ=255)                                                     cAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCGTACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcagc                                    <  1:4713896/100‑1 (MQ=255)                                                     cAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcagc                                    <  1:2825295/100‑1 (MQ=255)                                                     cAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcagc                                    <  1:2784591/100‑1 (MQ=255)                                                     cAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcagc                                    <  1:3919229/100‑1 (MQ=255)                                                     cAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcagc                                    <  1:2168949/100‑1 (MQ=255)                                                     cAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcagc                                    <  1:4426513/100‑1 (MQ=255)                                                        ttAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGCTTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGt                                 <  1:3145717/100‑1 (MQ=255)                                                        ttAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGt                                 <  1:1432702/100‑1 (MQ=255)                                                         tAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACcagcaggg                                <  1:3417554/100‑3 (MQ=255)                                                         tAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTa                                <  1:1970507/100‑1 (MQ=255)                                                         tAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTa                                <  1:5000027/100‑1 (MQ=255)                                                         tAAAGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTa                                <  1:1977731/100‑1 (MQ=255)                                                          aaaGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACTCCAGCAGTaa                               <  1:285415/100‑1 (MQ=255)                                                          aaaGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTag                               <  1:3269700/100‑2 (MQ=255)                                                          aaaGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTaa                               >  1:4391518/1‑100 (MQ=255)                                                           aaGTCTCTATTGAATCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTaaa                              <  1:2532800/100‑1 (MQ=255)                                                                         tCATTTTCAACATCAACGAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTAAATGGTACATTTTCtt                <  1:887609/100‑1 (MQ=255)                                                                                       aacgaGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTAAATGGTACATTTTCTTTTGCTAATTGTTGc  <  1:4691902/100‑1 (MQ=255)                                                                                       aacgaGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTAAATGGTACATTTTCTTTTGCTAATTGTTGc  <  1:1552577/100‑1 (MQ=255)                                                                                          |                                                                                                 TTCCCTCCAGAACCTACTGAAAAGATAACTTTATCGAATGGTTTAAACGTCTCAGTTAAAGTCTCTATTGAATCATTTTCAACATCAACAAGAATTGCTTTCATACCTTGTGATTTTAACGCATTAAGTTGATCTGATTGCCTAACACCAGCAGTAAATGGTACATTTTCTTTTGCTAATTGTTGC  >  NC_007793/2261697‑2261882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑