Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,234,368	C→T	E122E (GAG→GAA)	SAUSA300_RS11410 ←	peptide chain release factor 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,234,368	0	C	T	100.0%	219.9 / NA	76	E122E (GAG→GAA)	SAUSA300_RS11410	peptide chain release factor 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/62);  total (14/62)

CACTTTCAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCCTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCA  >  NC_007793/2234274‑2234461                                                                                               |                                                                                              cACTTTCAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTtcatc                                                                                          <  1:2279908/100‑1 (MQ=255)    tttCAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcacc                                                                                       <  1:4553464/100‑1 (MQ=255)    tttCAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcacc                                                                                       <  1:541904/100‑1 (MQ=255)    tttCAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcacc                                                                                       <  1:1941894/100‑1 (MQ=255)     ttCAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcacca                                                                                      <  1:4827708/100‑1 (MQ=255)     ttCAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcacca                                                                                      <  1:4582799/100‑1 (MQ=255)     tgctctccgatCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcacca                                                                                      <  1:4211400/90‑1 (MQ=255)     atctgACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcacca                                                                                      <  1:5012887/96‑1 (MQ=255)      tCAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcaccat                                                                                     <  1:596887/100‑2 (MQ=255)       cAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcaccgat                                                                                    <  1:5568695/100‑4 (MQ=255)       cAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGACTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcaccacg                                                                                    <  1:1702214/100‑2 (MQ=255)        atctgCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACct                                                                                   <  1:4702299/96‑1 (MQ=255)        aGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATcaccagat                                                                                   <  1:1304050/100‑4 (MQ=255)        aGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCaccacca                                                                                   <  1:3027395/100‑2 (MQ=255)        aGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACct                                                                                   <  1:5679114/100‑1 (MQ=255)         gACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctt                                                                                  <  1:4865082/100‑2 (MQ=255)         gACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctg                                                                                  <  1:2907262/100‑1 (MQ=255)         gACGCTTCTACTACTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctg                                                                                  >  1:4877156/1‑100 (MQ=255)          aCGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgt                                                                                 <  1:106761/100‑2 (MQ=255)          aCGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgc                                                                                 <  1:2515906/100‑1 (MQ=255)          aCGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgc                                                                                 <  1:3357113/100‑1 (MQ=255)           cttccgatctaTTTCGGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgct                                                                                <  1:1024539/92‑1 (MQ=255)           cttccgatctaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgct                                                                                <  1:4543009/92‑1 (MQ=255)           cttccgatctaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgct                                                                                <  1:5450505/92‑1 (MQ=255)           cttccgatctaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAACACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctggc                                                                                <  1:4144801/92‑3 (MQ=255)           cGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgcc                                                                                <  1:5626320/100‑2 (MQ=255)           atcTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgct                                                                                <  1:5284615/98‑1 (MQ=255)            gCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgctg                                                                               <  1:1290357/100‑1 (MQ=255)             tccgatctaTTTCGGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgctgc                                                                              <  1:2405731/94‑1 (MQ=255)             ctcttcctaTTTCAGTATTGAATCCTTGTGATTCAGCATACTTTGAATACATGCGCATTAAATCGCCAGCAAAAATCGCAGCTTCATCACCACctgctaa                                                                              <  1:1349512/94‑3 (MQ=255)             cTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgctgc                                                                              <  1:3239582/100‑1 (MQ=255)             cTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCACCACCACctgctgc                                                                              <  1:179719/100‑1 (MQ=255)               tCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgctgctg                                                                            >  1:569418/1‑100 (MQ=255)               tCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgctgctg                                                                            <  1:4766360/100‑1 (MQ=255)               tCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgctgctg                                                                            >  1:2388118/1‑100 (MQ=255)               cgatctaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgctgctg                                                                            <  1:812104/96‑1 (MQ=255)                ctactaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACctgctgccta                                                                           <  1:709723/100‑4 (MQ=255)                 tactaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTgctgctgct                                                                          <  1:2824344/100‑1 (MQ=255)                  tctaTGTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTgctgctgcta                                                                         <  1:1896472/99‑2 (MQ=255)                  actaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTgctgctgctt                                                                         <  1:2787543/100‑2 (MQ=255)                  actaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTgctgctgcta                                                                         <  1:5387556/100‑2 (MQ=255)                  actaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGctc                                                                         >  1:1757964/1‑100 (MQ=255)                  actaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGctc                                                                         >  1:3390902/1‑100 (MQ=255)                  actaTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGctc                                                                         <  1:5463670/100‑1 (MQ=255)                    taTTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCtt                                                                       <  1:878472/100‑1 (MQ=255)                     aTTTGAGTTTTGAATCCTTGTGATCCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTa                                                                      >  1:3608030/1‑100 (MQ=255)                         cAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTc                                                                  >  1:1283836/1‑100 (MQ=255)                          aggtttGAATCCTTGTGATTCAGCATACTTTGAATACATACGCAGTAAATCTCCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCt                                                                 <  1:406876/97‑1 (MQ=255)                           gTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTa                                                                <  1:2104072/100‑1 (MQ=255)                            ttttGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTAc                                                               <  1:3837701/100‑1 (MQ=255)                             tttGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTAct                                                              <  1:3532090/100‑2 (MQ=255)                             tttGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACa                                                              <  1:4394077/100‑1 (MQ=255)                             tttGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACa                                                              <  1:1762221/100‑1 (MQ=255)                              ttGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACag                                                             <  1:987224/100‑2 (MQ=255)                                      ttGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACggat                                                     <  1:3352181/100‑4 (MQ=255)                                             tCAGCATACTTTGAATACATACGCATTAAATCACCAGCCAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGtcat                                              >  1:5587548/1‑100 (MQ=255)                                             tCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGtcat                                              <  1:329657/100‑1 (MQ=255)                                              cAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGtcatc                                             >  1:174696/1‑100 (MQ=255)                                                         gAATACATACGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCtt                                  >  1:801298/1‑100 (MQ=255)                                                               atacGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTCTGTCATCATTAGGATCTTTAGGaa                            >  1:2042784/1‑100 (MQ=255)                                                                 tcGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATc                          >  1:1705950/2‑100 (MQ=255)                                                                 acGCATTAAATCACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGGATc                          >  1:5686111/1‑100 (MQ=255)                                                                            cACCAGCAAAAATCGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATAtttt               >  1:1685313/1‑100 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:4696209/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:4414177/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:954274/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:1216578/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:459244/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:659919/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:2212281/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:2234894/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:2409988/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:2413465/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:2700881/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:518191/100‑1 (MQ=255)                                                                                         cGCAGCTTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCa  <  1:3149977/100‑1 (MQ=255)                                                                                               |                                                                                              CACTTTCAGACGCTTCTACTATTTCAGTTTTGAATCCTTGTGATTCAGCATACTTTGAATACATACGCATTAAATCACCAGCAAAAATCGCAGCCTCATCACCACCTGCTGCTGCTCTTATTTCTACAATAACGTCTTTGTCATCATTAGGATCTTTAGGAATCAATAATATTTTAAGCTCTTCTTCA  >  NC_007793/2234274‑2234461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑