D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,230,311	C→T	G107E (GGA→GAA)	SAUSA300_RS11385 ←	serine hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,230,311	0	C	T	99.7%	1950.8 / ‑5.7	666	G107E (GGA→GAA)	SAUSA300_RS11385	serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (7/657); total (7/659)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

Only 100 of 667 total aligned reads displayed.

TCAACGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTCCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATGCTTTAGCACGATCA  >  NC_007793/2230212‑2230406

  aaCGAAATTGTAGAATTTACCCCTAAAATTAACAGGCGCCCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCt                                                                                               <  1:2678047/100‑1 (MQ=255)

  aaCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCt                                                                                               <  1:201432/100‑1 (MQ=255)

  aaCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCt                                                                                               <  1:198607/100‑1 (MQ=255)

  aaCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCt                                                                                               <  1:4090349/100‑1 (MQ=255)

  aaCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCt                                                                                               <  1:3280330/100‑1 (MQ=255)

  aaCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCt                                                                                               <  1:5306806/100‑1 (MQ=255)

  aaCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTCCt                                                                                               <  1:212676/100‑1 (MQ=255)

   aCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTa                                                                                              <  1:3879917/100‑1 (MQ=255)

   aCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTa                                                                                              <  1:3895115/100‑1 (MQ=255)

   aCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTa                                                                                              <  1:3958874/100‑1 (MQ=255)

   aCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTa                                                                                              <  1:1279270/100‑1 (MQ=255)

   aCGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTa                                                                                              <  1:719000/100‑1 (MQ=255)

    cGAAATTGTAGAATTTACCGCTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTaa                                                                                             <  1:4052251/100‑1 (MQ=255)

    cGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTaa                                                                                             <  1:5460670/100‑1 (MQ=255)

      aaaTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATg                                                                                           <  1:3409775/100‑1 (MQ=255)

       aaTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGc                                                                                          <  1:3536303/100‑1 (MQ=255)

       aaTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGc                                                                                          <  1:3159030/100‑1 (MQ=255)

       aaTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGc                                                                                          <  1:2741659/100‑1 (MQ=255)

       aaTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGc                                                                                          <  1:4907175/100‑1 (MQ=255)

       aaTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGc                                                                                          <  1:5055160/100‑1 (MQ=255)

       aaTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGc                                                                                          <  1:946341/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCGATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:2147814/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCGTGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:206126/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:4179098/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:4382825/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:3771008/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:3455574/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:533238/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:5610655/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:61386/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:3006365/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:1075476/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:1009713/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:1366384/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:2704210/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:2220204/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:245094/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:2577164/100‑1 (MQ=255)

        aTTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCGAAAACTGTGTCGCCCATTTCTAATGCa                                                                                         <  1:5021417/100‑1 (MQ=255)

         ttGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCaa                                                                                        <  1:4478813/100‑1 (MQ=255)

         ttGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCaa                                                                                        <  1:1532942/100‑1 (MQ=255)

         ttGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCaa                                                                                        <  1:1816267/100‑1 (MQ=255)

         ctGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCat                                                                                        <  1:4859064/99‑2 (MQ=255)

          tGTAGAATTTACCACTAAAATTAACAGGTGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAAc                                                                                       <  1:4593798/100‑1 (MQ=255)

          tGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACGTAAAACTGTGTCGCCCATTTCTAATGCaag                                                                                       <  1:2870699/100‑2 (MQ=255)

          tGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCaat                                                                                       <  1:837058/100‑2 (MQ=255)

          tGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCaag                                                                                       <  1:3191747/100‑2 (MQ=255)

          tGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCaaa                                                                                       <  1:2349037/100‑2 (MQ=255)

           gTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCaaga                                                                                      <  1:5788099/100‑3 (MQ=255)

            tAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCaggtc                                                                                     <  1:4227809/100‑5 (MQ=255)

            tAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTa                                                                                     <  1:1214137/100‑1 (MQ=255)

            tAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTa                                                                                     <  1:5727437/100‑1 (MQ=255)

            tAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTa                                                                                     <  1:5532122/100‑1 (MQ=255)

            tAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTa                                                                                     <  1:4819667/100‑1 (MQ=255)

            tAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTa                                                                                     <  1:27924/100‑1 (MQ=255)

            tAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATCTCTAATGCAACTa                                                                                     <  1:455986/100‑1 (MQ=255)

               aaTTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGt                                                                                  <  1:4724865/100‑1 (MQ=255)

                 tttACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTaa                                                                                <  1:4664175/100‑1 (MQ=255)

                 tttACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACGGAGTCGCCCATTTCTAATGCAACTAAGTaa                                                                                <  1:1405484/100‑1 (MQ=255)

                   tACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAAc                                                                              <  1:3351892/100‑1 (MQ=255)

                   tACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAAc                                                                              <  1:4505927/100‑1 (MQ=255)

                       aCTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGcc                                                                          <  1:2953883/100‑1 (MQ=255)

                       aCTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGcc                                                                          <  1:4615006/100‑1 (MQ=255)

                        cTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCa                                                                         <  1:4309392/100‑1 (MQ=255)

                        cTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCa                                                                         <  1:1456/100‑1 (MQ=255)

                         tAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCaa                                                                        <  1:1894182/100‑2 (MQ=255)

                         tAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCAt                                                                        <  1:2503386/100‑1 (MQ=255)

                         tAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCAt                                                                        <  1:2907582/100‑1 (MQ=255)

                          aaaaTTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCAta                                                                       <  1:5267931/100‑2 (MQ=255)

                          aaaaTTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATg                                                                       <  1:1742281/100‑1 (MQ=255)

                          aaaaTTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAACTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATg                                                                       <  1:159666/100‑1 (MQ=255)

                           aaaTTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAATTAAACAGCCAtag                                                                      <  1:4968070/100‑3 (MQ=255)

                           aaaTTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGt                                                                      <  1:2314699/100‑1 (MQ=255)

                           aaaTTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGt                                                                      <  1:240969/100‑1 (MQ=255)

                                aaCAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGca                                                                 <  1:889214/100‑2 (MQ=255)

                                  cAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTtg                                                               <  1:3620713/100‑1 (MQ=255)

                                   aGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTtgg                                                              <  1:3811926/100‑2 (MQ=255)

                                   aGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTtgg                                                              <  1:780572/100‑2 (MQ=255)

                                    ggCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTtgtg                                                             <  1:3045611/100‑1 (MQ=255)

                                    ggCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTtgtg                                                             <  1:5661518/100‑1 (MQ=255)

                                    ggCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTtgtg                                                             <  1:3973157/100‑1 (MQ=255)

                                     gcgcTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGa                                                            <  1:2654653/100‑1 (MQ=255)

                                     gcgcTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGa                                                            <  1:5432254/100‑1 (MQ=255)

                                                gtCAAGTGACCACCATGACGCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTgccagtt                                                 <  1:2239919/100‑7 (MQ=255)

                                                  cAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAAtgtgat                                               <  1:1858805/100‑3 (MQ=255)

                                                  cAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTggg                                               <  1:4268621/100‑2 (MQ=255)

                                                  cAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGt                                               <  1:1694768/100‑1 (MQ=255)

                                                    aGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTg                                             <  1:4427081/100‑1 (MQ=255)

                                                    aGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTg                                             <  1:3692910/100‑1 (MQ=255)

                                                     gTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGa                                            <  1:5209472/100‑1 (MQ=255)

                                                               tGACTCAAATTCATACCTAAAACTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACAt                                  <  1:4746562/100‑1 (MQ=255)

                                                                                 aaaaCTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATg                >  1:3254953/1‑100 (MQ=255)

                                                                                  aaaCTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATGc               >  1:1125529/1‑100 (MQ=255)

                                                                                     ctttgtCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATGCttt            <  1:4354787/97‑1 (MQ=255)

                                                                                     ccgatctgCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATGCttt            <  1:5129167/93‑1 (MQ=255)

                                                                                     cTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATGCttt            <  1:3922191/100‑1 (MQ=255)

                                                                                     cTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATGCttt            <  1:5681857/100‑1 (MQ=255)

                                                                                     cTGTGTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATGCttt            <  1:3212629/100‑1 (MQ=255)

                                                                                      tgtgTCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATGCtgag           <  1:536588/100‑4 (MQ=255)

                                                                                       gtgtCGCCCATTTCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATGCTTTAg          <  1:411762/100‑1 (MQ=255)

TCAACGAAATTGTAGAATTTACCACTAAAATTAACAGGCGCTCCATGTGTCAAGTGACCACCATGACTCAAATTCATACCTAAAACTGTGTCGCCCATTCCTAATGCAACTAAGTAAACAGCCATGTTCGCTTGTGAACCTGAATGTGGTTGAACATTGACATGTTCAGCTCCAAACAATGCTTTAGCACGATCA  >  NC_007793/2230212‑2230406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`