Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,158,122	T→G	C107W (TGT→TGG)	SAUSA300_RS10995 →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,158,122	0	T	G	100.0%	23.6 / NA	10	C107W (TGT→TGG)	SAUSA300_RS10995	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/1);  total (9/1)

CTTTAAATTCTAATGCAACAGTTTTTGAAGAAATGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGTTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACAATT  >  NC_007793/2158028‑2158211                                                                                               |                                                                                          cTTTAAATTCTAATGCAACAGTTTTTGAAGAAATGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGGTTATc                                                                                      <  1:4900790/100‑1 (MQ=255)            aaTGCAACAGTTTTTGAAGAAATGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGGTTATCAAAACATGCaa                                                                           >  1:1434160/1‑100 (MQ=255)            aaTGCAACAGTTTTTGAAGAAATGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGGTTATCAAAACATGCaa                                                                           >  1:2064167/1‑100 (MQ=255)                  aCAGTTTTTGAAGAAATGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGAtt                                                                     >  1:338963/1‑100 (MQ=255)                               taaTGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATAc                                                        >  1:3128535/2‑100 (MQ=255)                                                caaCATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGt                                       >  1:1849870/2‑100 (MQ=255)                                                 aaCATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTc                                      >  1:4494697/1‑100 (MQ=255)                                                          aaCGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGa                             >  1:646717/1‑100 (MQ=255)                                                                    aaGCTTAATCAAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCg                   >  1:4958216/1‑100 (MQ=255)                                                                                     aaaCAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACAAtt  >  1:280108/1‑100 (MQ=255)                                                                                               |                                                                                          CTTTAAATTCTAATGCAACAGTTTTTGAAGAAATGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGTTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACAATT  >  NC_007793/2158028‑2158211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑