Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,156,615	T→C	intergenic (‑5/+299)	SAUSA300_RS10985 ← / ← SAUSA300_RS10990	membrane protein/transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,156,615	0	T	C	100.0%	13.2 / NA	7	intergenic (‑5/+299)	SAUSA300_RS10985/SAUSA300_RS10990	membrane protein/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/6);  total (1/6)

TCTCGAAAGCCACCTGTTAAACAAAATCTTGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCTTAACCTCTCATTTCCGATAAGTTTAGTATGTATTATAGGTGATGATTTTCTTGTTTTCAACCTTATACACAA  >  NC_007793/2156519‑2156687                                                                                                 |                                                                         tccgatctgCCACCTGTTAAACAAAATCTTGTTCTTTGCATTACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCCTag                                                                       <  1:4551603/92‑2 (MQ=255)       aggCCACCTGTTAAACAAAATCTTGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCCTAACctcta                                                                 <  1:4091172/98‑2 (MQ=255)             ccTGTTAAACAAAATCGTGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCCTAACCTCTCAttcac                                                           <  1:32318/100‑4 (MQ=255)                   aaaCAAAATCTTGTTCTTTGCAGGACAAAACCTAAGAGACCCCCGAAAATTAAGCTACTAAAAAAAAGCCACATAATCCTATCCTCTCATTTCCGagcaa                                                     <  1:2493323/100‑5 (MQ=255)                             ttGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCCTAACCTCTCATTTCCGATAAGTTTAgtatgt                                           <  1:2545929/100‑1 (MQ=255)                                          gACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCCTAACCTCTCATTTCCGATAAGTTTAGTATGTATTATAGGtgatc                              <  1:5792674/100‑2 (MQ=255)                                                                      caGCCACTAAAAATCATCCACATAATCCTAACCTCTCATTTCCGATAAGTTTAGTATGTATTATAGGTGATGATTTTCTTGTTTTCAACCTTATACACaa  >  1:2254573/2‑100 (MQ=255)                                                                                                 |                                                                         TCTCGAAAGCCACCTGTTAAACAAAATCTTGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCTTAACCTCTCATTTCCGATAAGTTTAGTATGTATTATAGGTGATGATTTTCTTGTTTTCAACCTTATACACAA  >  NC_007793/2156519‑2156687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑