D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,148,597	T→A	F309I (TTT→ATT)	SAUSA300_RS10945 →	histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,148,597	0	T	A	99.7%	958.8 / ‑5.5	333	F309I (TTT→ATT)	SAUSA300_RS10945	histidine kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (326/6); minor base C (1/0); total (327/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 334 total aligned reads displayed.

AATGAATGCTATAAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATTTTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATTCTT  >  NC_007793/2148500‑2148683

 atgaatgCTATAAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTc                                                                                     >  1:3539227/1‑100 (MQ=255)

  tgaatgCTATAAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTcc                                                                                    >  1:552725/1‑100 (MQ=255)

  tgaatgCTATAAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTcc                                                                                    >  1:4195418/1‑100 (MQ=255)

     ctgCTATAAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGACAATTTTACGTGCACAAGAAATGAATATTCCGAt                                                                                 >  1:3727221/2‑100 (MQ=255)

          ttaAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTa                                                                            >  1:710057/2‑100 (MQ=255)

          ataAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTa                                                                            >  1:215100/1‑100 (MQ=255)

          ataAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTa                                                                            >  1:3586618/1‑100 (MQ=255)

           taAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTAt                                                                           >  1:4627139/1‑100 (MQ=255)

           aaaaaTTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTAt                                                                           >  1:1394340/2‑100 (MQ=255)

                 ctgctGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAAt                                                                     >  1:285670/5‑100 (MQ=255)

                 caaaTGGTATCGAGAATCTTAAAGTACGTGACATTAAAGGCTTAATTACTGCGAAAATTTTACGAGCACAAGAAATGAATATTCCGATTAGTATCGAAAt                                                                     >  1:4426694/2‑100 (MQ=255)

                  gtgtGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATa                                                                    >  1:2617849/4‑100 (MQ=255)

                  gcctGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATa                                                                    >  1:3488120/4‑100 (MQ=255)

                  acaTGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATa                                                                    >  1:2086639/3‑100 (MQ=255)

                  aaaTGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATa                                                                    >  1:768772/1‑100 (MQ=255)

                   caTGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAc                                                                   >  1:3690597/2‑100 (MQ=255)

                    gtGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAcc                                                                  >  1:2714398/2‑100 (MQ=255)

                    ctGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAcc                                                                  >  1:3116688/2‑100 (MQ=255)

                    ctGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAcc                                                                  >  1:3157640/2‑100 (MQ=255)

                    ctGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAAAAcc                                                                  >  1:5794532/2‑100 (MQ=255)

                    aTGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAcc                                                                  >  1:2466413/1‑100 (MQ=255)

                    aTGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAcc                                                                  >  1:3430407/1‑100 (MQ=255)

                     tGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAccc                                                                 >  1:1257623/1‑100 (MQ=255)

                     tGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAccc                                                                 <  1:3214281/100‑1 (MQ=255)

                     tGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAccc                                                                 >  1:5063090/1‑100 (MQ=255)

                     tGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAccc                                                                 >  1:1815806/1‑100 (MQ=255)

                     tGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATAccc                                                                 >  1:4476658/1‑100 (MQ=255)

                     tGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAACAccc                                                                 >  1:2545234/1‑100 (MQ=255)

                      ggTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCg                                                                >  1:866570/1‑100 (MQ=255)

                      ggTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCg                                                                >  1:935082/1‑100 (MQ=255)

                      ggTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCg                                                                >  1:3174217/1‑100 (MQ=255)

                      ggTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCg                                                                >  1:3364549/1‑100 (MQ=255)

                      ggTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCg                                                                >  1:5489632/1‑100 (MQ=255)

                      ggTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCg                                                                >  1:4267737/1‑100 (MQ=255)

                      ggTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCg                                                                >  1:433826/1‑100 (MQ=255)

                      ggTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCg                                                                >  1:4578368/1‑100 (MQ=255)

                      ggTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCg                                                                >  1:1657393/1‑100 (MQ=255)

                      ggTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCAAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCg                                                                >  1:692472/1‑100 (MQ=255)

                          gtgagaATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGa                                                            >  1:840450/3‑100 (MQ=255)

                           cGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGaa                                                           >  1:2063885/1‑100 (MQ=255)

                           cGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAAAAcccagatcg                                                           >  1:4332055/1‑94 (MQ=255)

                             agaATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGaagt                                                         >  1:5411123/1‑100 (MQ=255)

                                  cTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAagtaagta                                                    >  1:4499748/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGCTGaagatcggaag                                                  >  1:3294205/1‑92 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGaagatcggaag                                                  >  1:2979118/1‑92 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGaagatcggaag                                                  >  1:912730/1‑92 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGaagatcggaag                                                  >  1:3396008/1‑92 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:1165849/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:4758592/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:513330/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:51554/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:729153/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:1228058/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:2736597/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:2761527/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:4355668/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:2926389/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:1741575/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:4140152/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:3945607/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:3014426/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:269779/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:2642835/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:1960362/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:2021184/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:2044346/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:2222390/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTCTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:4084703/1‑100 (MQ=255)

                                    tAAAGTACGTGAAATTAAAGGCTTAATTACCGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGc                                                  >  1:2332913/1‑100 (MQ=255)

                                     aaaGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCa                                                 >  1:2787087/1‑100 (MQ=255)

                                     aaaGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCa                                                 >  1:137046/1‑100 (MQ=255)

                                                   tAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGaacaga                                   >  1:3899762/1‑96 (MQ=255)

                                                     aaGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGAt                                 >  1:1299572/1‑100 (MQ=255)

                                                      aGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTCGAATAAGATc                                >  1:1530313/1‑100 (MQ=255)

                                                           tAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGAttt                           >  1:3842872/1‑100 (MQ=255)

                                                           tAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATA‑GATCggaaga                          >  1:4892360/1‑95 (MQ=255)

                                                               tACTGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACGTGAATA‑GATCggaagagcac                      >  1:1596667/1‑91 (MQ=255)

                                                                  tGCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGc                    <  1:1073872/100‑1 (MQ=255)

                                                                  ggCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGc                    <  1:5570730/99‑1 (MQ=255)

                                                                   gCGAAAATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCa                   >  1:5254288/1‑100 (MQ=255)

                                                                            ttACGTGCACAAGAAAGGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGta          >  1:1874460/1‑100 (MQ=255)

                                                                             tACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtat         >  1:4997856/1‑100 (MQ=255)

                                                                              ccGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtatt        >  1:5204569/2‑100 (MQ=255)

                                                                              ccGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtatt        >  1:3059382/2‑100 (MQ=255)

                                                                              ccGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtatt        >  1:1433684/2‑100 (MQ=255)

                                                                              aCGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAgatcggaagag        >  1:574080/1‑90 (MQ=255)

                                                                              aCGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtatt        >  1:501052/1‑100 (MQ=255)

                                                                              aCGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtatt        >  1:2424429/1‑100 (MQ=255)

                                                                              aCGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtatt        >  1:2378801/1‑100 (MQ=255)

                                                                               cGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtatta       >  1:150999/1‑100 (MQ=255)

                                                                                gTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtattat      >  1:4711819/1‑100 (MQ=255)

                                                                                gTGCACAAGAAATGAATATTCAGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtattat      >  1:1852640/1‑100 (MQ=255)

                                                                                ctGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGtattat      >  1:1704988/2‑100 (MQ=255)

                                                                                  gCACAAGAAATGAATATTCCGATTAGTATCGTAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATTc    >  1:4310878/1‑100 (MQ=255)

                                                                                  gCACAAGAAATGAATATTCCGATTAGTATCGATATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGCCGCAGTATTGGTATTATTc    >  1:5361204/1‑100 (MQ=255)

                                                                                  gCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTCACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATTc    >  1:1041746/1‑100 (MQ=255)

                                                                                  gCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATTc    >  1:5668944/1‑100 (MQ=255)

                                                                                  gCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATTc    >  1:4810581/1‑100 (MQ=255)

                                                                                  gCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATTc    >  1:403863/1‑100 (MQ=255)

                                                                                  gCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATTc    >  1:2252026/1‑100 (MQ=255)

AATGAATGCTATAAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAAATTTTACGTGCACAAGAAATGAATTTTCCGATTAGTATCGAAATACCCGATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATTCTT  >  NC_007793/2148500‑2148683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`