Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,146,812	T→A	intergenic (‑106/‑130)	SAUSA300_RS10930 ← / → SAUSA300_RS10935	delta‑hemolysin/accessory gene regulator protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,146,812	0	T	A	100.0%	66.2 / NA	25	intergenic (‑106/‑130)	SAUSA300_RS10930/SAUSA300_RS10935	delta‑hemolysin/accessory gene regulator protein B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/13);  total (12/13)

CTGTGATCTAGTTATATTAAAACATGCTAAAAGCATTTATTTTCCAATTTTTCTTAACTAGTCGTTTTTTATTCTTAACTGTAATTTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCAATATAATGATAAAAGATTGTACT  >  NC_007793/2146728‑2146905                                                                                     |                                                                                              ctGTGATCTAGTTATATTAAAACATGCTAAAAGCATTTATTTTCCAATTTTTCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTaaa                                                                                >  1:778133/1‑100 (MQ=255) ctGTGATCTAGTTATATTAAAACATGCTAAAAGCATTTATTTTCCAATTTTTCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTaaa                                                                                >  1:1325755/1‑100 (MQ=255) ctGTGATCTAGTTATATTAAAACATGCTAAAAGCATTTATTTTCCAATTTTTCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTaaa                                                                                >  1:1357627/1‑100 (MQ=255) ctGTGATCTAGTTATATTAAAACATGCTAAAAGCATTTATTTTCCAATTTTTCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTaaa                                                                                >  1:5379920/1‑100 (MQ=255) ctGTGATCTAGTTATATTAAAACATGCTAAAAGCATTTATTTTCCAATTTTTCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTaaa                                                                                >  1:2721398/1‑100 (MQ=255)          aGTTATATTAAAACATGCTAAAAGCATTTATTTTCCAATTTTTCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAAt                                                                       >  1:5097361/1‑100 (MQ=255)          aGTTATATTAAAACATGCTAAAAGCATTTATTTTCCAATTTTTCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTCTTTATGTTAAAATATTAAAt                                                                       >  1:3284046/1‑100 (MQ=255)                             aaaaGCATTTATTTTCCAATTTTTCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGt                                                    >  1:493656/1‑100 (MQ=255)                                                 ttttCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAg                                <  1:2328047/100‑1 (MQ=255)                                                   ttccgatcTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGtt                              <  1:2910001/93‑1 (MQ=255)                                                   ttCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGtt                              <  1:953279/100‑1 (MQ=255)                                                   ttCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGtt                              <  1:604505/100‑1 (MQ=255)                                                   ttCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGtt                              >  1:4169584/1‑100 (MQ=255)                                                   ttCTTAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAACACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAgct                              <  1:5005807/100‑3 (MQ=255)                                                       tAACTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGc                          >  1:3062952/1‑100 (MQ=255)                                                       gatcTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGc                          <  1:931653/97‑1 (MQ=255)                                                        atcTAGTCGCTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCa                         <  1:1237166/98‑1 (MQ=255)                                                         aCTAGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCaa                        <  1:1682245/100‑1 (MQ=255)                                                            aGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCAatat                     >  1:3557313/1‑100 (MQ=255)                                                            aGTCGTTTTTTATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCAatat                     >  1:1384432/1‑100 (MQ=255)                                                                   ttttATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCAATATAATGATa              <  1:1288198/100‑1 (MQ=255)                                                                     ttATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCAATATAATGATaag            <  1:3404555/100‑2 (MQ=255)                                                                     ttATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCAATATAATGATaag            <  1:236269/100‑2 (MQ=255)                                                                     ttATTCTTAACTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCAATATAATGATaag            <  1:5338276/100‑2 (MQ=255)                                                                               cTGTAAATTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCAATATAATGATAAGAGATTGTACt  <  1:3095114/100‑1 (MQ=255)                                                                                     |                                                                                              CTGTGATCTAGTTATATTAAAACATGCTAAAAGCATTTATTTTCCAATTTTTCTTAACTAGTCGTTTTTTATTCTTAACTGTAATTTTTTTTATGTTAAAATATTAAATACAAATTACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCAATATAATGATAAAAGATTGTACT  >  NC_007793/2146728‑2146905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑