Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,143,811	G→C	P66A (CCT→GCT)	SAUSA300_RS10915 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,143,811	0	G	C	100.0%	70.1 / NA	28	P66A (CCT→GCT)	SAUSA300_RS10915	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (0/28);  total (0/28)

TTGTTTGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGGTGTCCCAGTCTCATTATTATTCATTTCACTATTTACTGATGGTGAAGTTATATTATGATTCAAATTAGTAGTTGGTTTTTCTCCATTTA  >  NC_007793/2143717‑2143900                                                                                               |                                                                                          ttgttTGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGctgtga                                                                                      <  1:1411887/100‑3 (MQ=255)  tgttTGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTccc                                                                                     <  1:539673/100‑1 (MQ=255)  tgttTGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTccc                                                                                     <  1:3004290/100‑1 (MQ=255)     ttGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAgg                                                                                  <  1:3105848/100‑2 (MQ=255)     ttGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGt                                                                                  <  1:4127402/100‑1 (MQ=255)     ttGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGt                                                                                  <  1:4643325/100‑1 (MQ=255)     ttGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGt                                                                                  <  1:5498801/100‑1 (MQ=255)     ttGAGTCTGGCTACACATTATTCGAATAAGGATTAGCATCACGACTATACGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAgg                                                                                  <  1:2738230/100‑2 (MQ=255)      tGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTTGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGtc                                                                                 <  1:1143448/100‑1 (MQ=255)      tGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGtc                                                                                 <  1:5731701/100‑1 (MQ=255)       gAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAgcgg                                                                                <  1:5700879/100‑4 (MQ=255)       gAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGtcc                                                                                <  1:3876887/100‑2 (MQ=255)        aGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGtcta                                                                               <  1:5174488/100‑2 (MQ=255)           cTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGTCTCatt                                                                            <  1:670754/100‑1 (MQ=255)            tGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGtctcccat                                                                           <  1:771596/100‑5 (MQ=255)             ggCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGTCTCattac                                                                          <  1:5255360/100‑2 (MQ=255)              gCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGTCTCattatt                                                                         <  1:5630103/100‑1 (MQ=255)                   acaTTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGTCTCattagccatgt                                                                    <  1:2658812/100‑8 (MQ=255)                          tCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGTCTCATTATTATTCATTTCACt                                                             <  1:4919903/100‑1 (MQ=255)                          tCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGTCTCATTATTATTCATTTCACt                                                             <  1:363492/100‑1 (MQ=255)                          tCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTCGATTTGATTCGTGAGCTGTCCCAGTCTCATTATTATTCATTTCACt                                                             <  1:5757625/100‑1 (MQ=255)                            gAATCAGGATTAGCATCGCGTCTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGTGCTGTGCCAGTCTCATTATTATTCACTTCACTAt                                                           <  1:5092449/100‑1 (MQ=255)                              aTCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGTCTCATTATTATTCATTTCACTAttt                                                         <  1:791232/100‑1 (MQ=255)                                cAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGTCTCATTATTATTCATTTCACTATTTAc                                                       <  1:5731987/100‑1 (MQ=255)                                  ggATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATCCGTGAGCTGTCCCAGTCTCATTATTATTCATTTCACTATTTACTg                                                     <  1:3201345/100‑1 (MQ=255)                                        gCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGTCTCATTATTATTCATTTCACTATTTACTGATGGtc                                               <  1:4553346/100‑2 (MQ=255)                                                                 cTTCATTACCCGTTTGATTTGATTCGTGAGCTGTCCCAGTCTCATTATTATTCATTTCACTATTTACTGATGGTGAAGTTATATTATGATTCAAATtagt                      <  1:5417294/100‑1 (MQ=255)                                                                             tttgatttgaTTCGTGAGCTGTCCCAGTCTCATTATTATTCATTTCACTATTTACTGATGGTGAAGTTATATTATGATTCAAATTAGTAGTTGGttctgt          <  1:4271450/100‑5 (MQ=255)                                                                                     gattCGTGAGCTGTCCCAGTCTCATTATTATTCATTTCACTATTTACTGATGGTGAAGTTATATTATGATTCAAATTAGTAGTTGGTTTTTCTCCATTTa  <  1:4838592/100‑1 (MQ=255)                                                                                               |                                                                                          TTGTTTGAGTCTGGCTTCACATTATTCGAATCAGGATTAGCATCACGACTATTCGAACCTGTTCCTTCATTACCCGTTTGATTTGATTCGTGAGGTGTCCCAGTCTCATTATTATTCATTTCACTATTTACTGATGGTGAAGTTATATTATGATTCAAATTAGTAGTTGGTTTTTCTCCATTTA  >  NC_007793/2143717‑2143900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑