Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,133,658	G→T	P27T (CCA→ACA)	SAUSA300_RS10860 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,133,658	0	G	T	100.0%	80.9 / NA	28	P27T (CCA→ACA)	SAUSA300_RS10860	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/28);  total (0/28)

AATTTGAGACATGTCATCACATGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGGTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAACCAGTTGCTTCATTTAATCACTCCTTAA  >  NC_007793/2133569‑2133751                                                                                          |                                                                                              aaTTTGAGACATGTCATCACATGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTGTGATAGTGCGTCTTTTATTATTGTTttttcgtta                                                                                     <  1:4479191/100‑2 (MQ=255)     tGAGACATGTCATCACATGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTtttcgttttcgt                                                                                 <  1:3280170/100‑1 (MQ=255)      gagaCATGTCATCACATGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTa                                                                                <  1:3460908/100‑1 (MQ=255)        gaCATGTCATCACATGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTAct                                                                              <  1:2799057/100‑1 (MQ=255)            tGTCATCACATGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTAccacac                                                                          <  1:2903960/100‑6 (MQ=255)             gTCATCACATGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTAccacagg                                                                         <  1:3145141/100‑7 (MQ=255)                atcaCATGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGcc                                                                      <  1:1446700/100‑2 (MQ=255)                atcaCATGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCt                                                                      <  1:3783374/100‑1 (MQ=255)                    caTGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAg                                                                  <  1:731132/100‑1 (MQ=255)                      tGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCt                                                                <  1:1254797/100‑1 (MQ=255)                      tGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCt                                                                <  1:5177008/100‑1 (MQ=255)                       gTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTa                                                               <  1:2698779/100‑1 (MQ=255)                        tAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTAt                                                              <  1:2352694/100‑1 (MQ=255)                        tAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTAt                                                              <  1:2857451/100‑1 (MQ=255)                         aaCATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATc                                                             <  1:4352610/100‑1 (MQ=255)                          aCATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCa                                                            <  1:1229806/100‑1 (MQ=255)                                                   cATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAAt                                   <  1:3485993/100‑1 (MQ=255)                                                          tatTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAAcc                            <  1:206735/100‑1 (MQ=255)                                                          tatTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAAcc                            <  1:4447052/100‑1 (MQ=255)                                                          gatTTTTTTTGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAAcc                            <  1:1821879/99‑1 (MQ=255)                                                            ttttttttGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAACCAg                          <  1:1169831/100‑1 (MQ=255)                                                              ttttttGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAACCAGtt                        <  1:5358814/100‑1 (MQ=255)                                                              ttttttGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAACCAGta                        <  1:5432285/100‑2 (MQ=255)                                                                 tttGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAACCAGTTGCt                     <  1:749092/100‑1 (MQ=255)                                                                  ttGATAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAACCAGTTGCta                    <  1:2664150/100‑2 (MQ=255)                                                                     aTAGTGCGTCTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAACCAGTTGCTTCAt                 <  1:1722275/100‑1 (MQ=255)                                                                              cTTTTATTATTGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATGAAAGGAATTAACATTGATTCGGGAATTCCAACCAGTTGCTTCATTTAATCACt        <  1:4410384/100‑1 (MQ=255)                                                                                    ttattGTTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAACCAGTTGCTTCATTTAATCACTCCTTaa  <  1:2627338/100‑1 (MQ=255)                                                                                          |                                                                                              AATTTGAGACATGTCATCACATGTAACATTTTTGTACATATCATCTAAACCATCAAATATTTTTTTTGATAGTGCGTCTTTTATTATTGGTTTTTCGTTTTCGTACTCTTTTGCTCGAGCTATCAAAGGAATTAACATTGATTCGGGAATTCCAACCAGTTGCTTCATTTAATCACTCCTTAA  >  NC_007793/2133569‑2133751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑