Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,127,837	G→A	V44V (GTG→GTA)	SAUSA300_RS10840 →	phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,127,837	0	G	A	100.0%	50.1 / NA	18	V44V (GTG→GTA)	SAUSA300_RS10840	phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/17);  total (1/17)

AAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTGAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCTACTCATCAACTGTTGCAG  >  NC_007793/2127766‑2127928                                                                        |                                                                                            aaaaaTAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTg                                                                 <  1:5751766/100‑1 (MQ=255)  aaaaTAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGt                                                                <  1:2005918/100‑1 (MQ=255)   aaaTAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTa                                                               <  1:247325/100‑1 (MQ=255)    aataatGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTAc                                                              <  1:1535004/100‑1 (MQ=255)       aatGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACtgc                                                           <  1:5100971/100‑3 (MQ=255)            tgTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGt                                                      <  1:5239332/100‑1 (MQ=255)                    tatTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATc                                              <  1:2859112/100‑1 (MQ=255)                         aaTGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGt                                         <  1:1634823/100‑1 (MQ=255)                         aaTGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGt                                         <  1:5755396/100‑1 (MQ=255)                            gAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATAGCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTggt                                      <  1:307884/100‑2 (MQ=255)                                 aTTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTGCTCGGCCGTTCGCAATCAGGGTGGGACaat                                 <  1:2536183/100‑2 (MQ=255)                                       tAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGag                           <  1:156152/100‑2 (MQ=255)                                        aaTGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAg                          <  1:3717231/100‑1 (MQ=255)                                              gCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCt                    <  1:2836524/100‑1 (MQ=255)                                                  cAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCTACtc                >  1:3043509/1‑100 (MQ=255)                                                            attaAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCTACTCATCAACTGtt      <  1:3637859/100‑1 (MQ=255)                                                              taAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCTACTCATCAACTGTTGc    <  1:2840836/100‑1 (MQ=255)                                                                aGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCTACTCATCAACTGTTGctt  <  1:4736229/100‑3 (MQ=255)                                                                        |                                                                                            AAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTGAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCTACTCATCAACTGTTGCAG  >  NC_007793/2127766‑2127928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑