Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,127,455	T→C	S282P (TCA→CCA)	SAUSA300_RS10835 →	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,127,455	0	T	C	100.0%	27.4 / NA	12	S282P (TCA→CCA)	SAUSA300_RS10835	integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/1);  total (11/1)

TGATGGTTACATTTTCAAAGAAGCCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATATCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATCTATACATTACAT  >  NC_007793/2127364‑2127531                                                                                            |                                                                             tgatgGTTACATTTTCAAAGAAGGCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAattatt                                                                      >  1:3242615/1‑100 (MQ=255)  gctgGTTACATTTTCAAAGAAGGCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAAttatta                                                                     >  1:1410035/3‑100 (MQ=255)  gatgGTTACATTTTCAAAGAAGGCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAAttatta                                                                     >  1:1393412/1‑100 (MQ=255)        tACATTTTCAAAGAAGGCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATcgc                                                               >  1:5360360/1‑100 (MQ=255)                  aagaagGCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATcgcacacagatcg                                                     >  1:135394/1‑95 (MQ=255)                   attaggcGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGa                                                    >  1:860774/7‑100 (MQ=255)                           gATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACTCa                                            <  1:783512/100‑1 (MQ=255)                                ataATTACCCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACTCATTGtt                                       >  1:5144951/1‑100 (MQ=255)                                ataATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACTCATTGtt                                       >  1:3236827/1‑100 (MQ=255)                                                         acaacaaaaaaTCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATc              >  1:391317/9‑100 (MQ=255)                                                                     cAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATCTAtacattacat  >  1:1069816/1‑100 (MQ=255)                                                                     cAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATCTAtacattacat  >  1:4514030/1‑100 (MQ=255)                                                                                            |                                                                             TGATGGTTACATTTTCAAAGAAGCCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATATCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATCTATACATTACAT  >  NC_007793/2127364‑2127531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑