| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 2,127,281 | G→A | D224N (GAT→AAT) | SAUSA300_RS10835 → | integrase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 2,127,281 | 0 | G | A | 93.8% | 35.1 / ‑4.7 | 16 | D224N (GAT→AAT) | SAUSA300_RS10835 | integrase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (14/1); total (15/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
TACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCGATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACATTTGCAATTAGCTATGAT > NC_007793/2127190‑2127367 | caCGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTcc > 1:4784503/2‑100 (MQ=255) gtggtgCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTAt > 1:1358935/1‑100 (MQ=255) aaGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTaaa > 1:3072693/1‑100 (MQ=255) cAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATggct < 1:576924/100‑3 (MQ=255) cAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGAc > 1:2195416/1‑100 (MQ=255) taCCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACg > 1:1017813/2‑100 (MQ=255) caGTGAAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCAt > 1:1945808/2‑100 (MQ=255) caGTGAAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCAt > 1:366274/2‑100 (MQ=255) gAAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCATAAGt > 1:1268257/1‑100 (MQ=255) aaaCACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACa > 1:821719/1‑100 (MQ=255) cTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACATTTGc > 1:2830124/1‑100 (MQ=255) atatTTATTGACGAGCGAAAAACCGATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACATTTGCaa > 1:5324543/1‑100 (MQ=255) attGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACATTTGCAATTAGCt > 1:1038778/1‑100 (MQ=255) ttGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACATTTGCAATTAGCTa > 1:1667941/1‑100 (MQ=255) tGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACATTTGCAATTAGCTat > 1:433431/1‑100 (MQ=255) gtggCGAAAAACCAATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACATTTGCAATTAGCTatgat > 1:4672013/4‑100 (MQ=255) | TACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCGATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACATTTGCAATTAGCTATGAT > NC_007793/2127190‑2127367 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |