Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,126,809	G→A	T66T (ACG→ACA)	SAUSA300_RS10835 →	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,126,809	0	G	A	100.0%	53.2 / NA	21	T66T (ACG→ACA)	SAUSA300_RS10835	integrase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/2);  total (19/2)

TCTGCTGGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACGTATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATTATAGAAATGCATTACAACAT  >  NC_007793/2126714‑2126869                                                                                                |                                                             gctgctgGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACAtata                                                          >  1:3130917/2‑100 (MQ=255)   acttgGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATaaa                                                        >  1:860894/4‑100 (MQ=255)    gctgGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAaac                                                       >  1:550645/1‑100 (MQ=255)    gctgGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAaac                                                       >  1:155101/1‑100 (MQ=255)    gctgGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAaac                                                       >  1:3486349/1‑100 (MQ=255)    gctgGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAaac                                                       >  1:3646274/1‑100 (MQ=255)    gctgGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTAAATAACGAATGAAGAATACTTAGAAAATTGGATTAAAACAGATaaaa                                                       >  1:5706732/1‑99 (MQ=255)    cctgGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAaac                                                       >  1:2133932/2‑100 (MQ=255)      tgGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAaacaa                                                     >  1:486773/1‑100 (MQ=255)       ggACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAaacaac                                                    >  1:963373/1‑100 (MQ=255)       ggACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAaacaac                                                    >  1:262465/1‑100 (MQ=255)       ggACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAaacaac                                                    >  1:2681530/1‑100 (MQ=255)       ggACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAaacaac                                                    >  1:4183196/1‑100 (MQ=255)            tgACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAAACAACCtgtt                                               >  1:2083734/2‑100 (MQ=255)            aGACAAGTTAAATTTTTTAAGAAGTGGTTTTCCTCTCGATAACTATATAACTCTTGAAGAATACTTCGAAGATTTGATTAAAACATATAAACAACCtgtt                                               >  1:2479577/1‑100 (MQ=255)                             tAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAAACAACCTGTTGTTAAAGAAAAtaccta                              >  1:239833/1‑100 (MQ=255)                                       ttttAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATtag                    <  1:4181618/100‑2 (MQ=255)                                       ttttAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAAACAACCGGTTGTTAAAGAAAATACCTACCGTCATtat                    <  1:5229385/100‑1 (MQ=255)                                                        atataACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATTATAGAAATGCATTacaaca   >  1:156583/1‑100 (MQ=255)                                                        atataACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATTATAGAAATGCATTacaaca   >  1:1868969/1‑100 (MQ=255)                                                         tataACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATTATAGAAATGCATTACAACat  >  1:996698/1‑100 (MQ=255)                                                                                                |                                                             TCTGCTGGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACGTATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATTATAGAAATGCATTACAACAT  >  NC_007793/2126714‑2126869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑