Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,126,660	T→C	Y17H (TAT→CAT)	SAUSA300_RS10835 →	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,126,660	0	T	C	100.0%	41.7 / NA	17	Y17H (TAT→CAT)	SAUSA300_RS10835	integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/6);  total (11/6)

TAGCCCGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGTATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAGTTAAATGAGTTA  >  NC_007793/2126564‑2126743                                                                                                 |                                                                                    tAGCCCGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATg                                                                                  >  1:682272/1‑100 (MQ=255)  aGCCCGCCTACCCTTATTATTTTTTGCCAATTTTGAGGCGGGAACGCATGAAAAAACGTTGTTACGATGGAAAAAAATGGCAATATGAATTTAAGCATGa                                                                                 >  1:1846596/1‑100 (MQ=38)  aGCCCGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGa                                                                                 >  1:2035422/1‑100 (MQ=255)  aGCCCGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGa                                                                                 >  1:5314066/1‑100 (MQ=255)   tcccGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGaa                                                                                >  1:2025399/2‑100 (MQ=255)    cccGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAg                                                                               >  1:5213524/1‑100 (MQ=255)    cccGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAg                                                                               >  1:2828362/1‑100 (MQ=255)      cGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATAGCAATATGAATTTAAGCATGAAgat                                                                             >  1:3672737/1‑98 (MQ=255)       gCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGaa                                                                            >  1:3410374/1‑100 (MQ=255)         cTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAGAAAATGGCAATATGAATTTAAGCATGAAGGaaaa                                                                          <  1:2977641/100‑1 (MQ=255)                              aaTTTGGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGtttt                                                     <  1:4644977/100‑1 (MQ=255)                                     aggaggGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACaa                                              <  1:3856767/100‑1 (MQ=255)                                                        cacGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTctgc                           <  1:3033989/100‑1 (MQ=255)                                                          cGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTctgctg                         <  1:5425795/100‑1 (MQ=255)                                                              gttACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACt                     <  1:4446395/100‑1 (MQ=255)                                                                tACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCCGGACTAg                   >  1:5759175/1‑100 (MQ=255)                                                                                 ccAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAGTTAAATGAGTTa  >  1:552580/2‑100 (MQ=255)                                                                                                 |                                                                                    TAGCCCGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGTATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAGTTAAATGAGTTA  >  NC_007793/2126564‑2126743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑