Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,125,650	T→C	intergenic (+20/‑58)	SAUSA300_RS10825 → / → SAUSA300_RS10830	hypothetical protein/toxin MazF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,125,650	0	T	C	100.0%	10.3 / NA	6	intergenic (+20/‑58)	SAUSA300_RS10825/SAUSA300_RS10830	hypothetical protein/toxin MazF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/0);  total (6/0)

AATGCGCGTAAATACTTGGAATTTATTTCACAAAAAATAGATGGCGATAAGTTAACCAAAGAAGATTCATTATGATTTTACTAATC‑AAAAAACGTCTACAAGTGTAGACGTTGA  >  NC_007793/2125556‑2125669                                                                                                |                    aaTGCGCGTAAATACTTGGAATTTATTTCACAAAAATTAGATGGCGATAAGTTAACCAAAGAAGATTCATTATGATTTTACTAAAC‑AAAAAACGCCTAct                >  1:3470874/1‑99 (MQ=255)    gcgcGTAAATACTTGGAATTTATTTCACAAAAATTAGATGGCGATAAGTTAACCAAAGAAGATTCATTATGATTTTACTAAAC‑AAAAAACGCCTACTAgt             >  1:1873586/1‑100 (MQ=255)        gTAAATACTTGGAATTTATTTCACAAAAATTAGATGGCGATAAGTTAACCAAAGAAGATTCATTATGATTTTACTAAACAAAAAAACGCCTACTAgtgtg          >  1:1192966/1‑99 (MQ=255)          caaTACTTGGAATTTATTTCACAAAAATTAGATGGCGATAAGTTAACCAAAGAAGATTCATTATGATTTTACTAAACAAAAAAACGCCTACTAgtgtgaa        >  1:5339638/2‑97 (MQ=255)             caCTTGGAATTTATTTCACAAAAATTAGATGGCGATAAGTTAACCAAAGAAGATTCATTATGATTTTACTAAAC‑AAAAAACGCCTACTAgtgtgaaaacg    >  1:5324164/2‑93 (MQ=255)               cTTGGAATTTATTTCACAAAAATTAGATGGCGATAAGTTAACCAAAGAAGATTCATTATGATTTTACTAAAC‑AAAAAACGCCTACTAgtgtgaaaacgta  >  1:225185/1‑91 (MQ=255)                                                                                                |                    AATGCGCGTAAATACTTGGAATTTATTTCACAAAAAATAGATGGCGATAAGTTAACCAAAGAAGATTCATTATGATTTTACTAATC‑AAAAAACGTCTACAAGTGTAGACGTTGA  >  NC_007793/2125556‑2125669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑