Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,122,423	A→G	D45G (GAT→GGT)	SAUSA300_RS10790 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,122,423	0	A	G	100.0%	24.1 / NA	11	D45G (GAT→GGT)	SAUSA300_RS10790	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/6);  total (5/6)

CTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTATTTCGGTAGTGATGAATCCTCATTTAACGCTTTCTTTTTAGATTTTAATTTTGAAGACTATATCGATGCAGTTCTTGAATTAAAGCATAGAGATTTTGTAATTGCTTCTGCTGAAGATGGCGGTTTTCTTG  >  NC_007793/2122338‑2122512                                                                                      |                                                                                          cTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTATTTCGGTAGTGATGAATCCTCATTTAACGCTTTCTTTTTAGGTTTTAATTTTGAAg                                                                             <  1:3496997/100‑1 (MQ=255)   tGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTATTTCGGTAGTGATGAATCCTCATTTAACGCTTTCTTTTTAGGTTTTAATTTTGAAGAc                                                                           >  1:2243969/1‑100 (MQ=255)             gACGAAGGTATGTCTAAACGTTCTGCATCTTATTTCGGTAGTGATGAATCCTCATTTAACGCTTTCTTTTTAGGTTTTAATTTTGAAGACTATATCGATg                                                                 >  1:175257/1‑100 (MQ=255)                                     gCATCTTATTTCGGTAGTGATGAATCCTCATTTAACGCTTTCTTTTTAGGTTTTAATTTTGAAGACTATATCGATGCAGTTCTTGAATTAAAGCATagag                                         >  1:2671700/1‑100 (MQ=255)                                           tATTTCGGTAGTGATGAATCCTCATTTAACGCTTTCTTTTTAGGTTTTAATTTTGAAGACTATATCGATGCAGTTCTTGAATTAAAGCATAGAGAtcgga                                   >  1:3444767/1‑96 (MQ=255)                                           tATTTCGGTAGTGATGAATCCTCATTTAACGCTTTCTTTTTAGGTTTTAATTTTGAAGACTATATCGATGCAGTTCTTGAATTAAAGCATAGAGATTTTg                                   >  1:1820163/1‑100 (MQ=255)                                              ttCGGTAGTGATGAATCCTCATTTAACGCTTGCTTTTTAGGTTTTAATTTTGAAGACTATATCGATGCAGTTCTTGAATTAAAGCATAGAGATTTTGTag                                <  1:36442/100‑2 (MQ=255)                                                cGGTAGTGATGAATCCTCATTTAACGCTTTCTTTTTAGGTTTTAATTTTGAAGACTATATCGATGCAGTTCTTGAATTAAAGCATAGAGATTTTGTAAtt                              <  1:271447/100‑1 (MQ=255)                                                                     tAACGCTTTCTTTTTAGGTTTTAATTTTGAAGACTATATCGATGCAGTTCTTGAATTAAAGCATAGAGATTTTGTAATTGCTTCTGCTGAAGATGGCGGt         <  1:3477593/100‑1 (MQ=255)                                                                      aaCGCTTTCTTTTTAGGTTTTAATTTTGAAGACTATATCGATGCAGTTCTTGAATTAAAGCATAGAGATTTTGTAATTGCTTCTGCTGAAGATGGCGGtt        <  1:1363879/100‑1 (MQ=255)                                                                            ttctttTTAGGTTTTAATTTTGAAGACTATATCGATGCAGTTCTTGAATTAAAGCATAGAGATTTTGTAATTGCTTCTGCTGAAGATGGCGGTTTTCTTg  <  1:4200545/100‑1 (MQ=255)                                                                                      |                                                                                          CTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTATTTCGGTAGTGATGAATCCTCATTTAACGCTTTCTTTTTAGATTTTAATTTTGAAGACTATATCGATGCAGTTCTTGAATTAAAGCATAGAGATTTTGTAATTGCTTCTGCTGAAGATGGCGGTTTTCTTG  >  NC_007793/2122338‑2122512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑