Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,122,296	A→G	N3D (AAT→GAT)	SAUSA300_RS10790 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,122,296	0	A	G	100.0%	53.9 / NA	21	N3D (AAT→GAT)	SAUSA300_RS10790	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/8);  total (13/8)

ACATTTCTCCGATGTTCGAATTTGTTTGTAATGCTTGCATATTGTTTATGCTCCTTTCTGCTATACTCCTATTAAGGAGGTGAATGACTTATGACTAATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTATTTCGGTA  >  NC_007793/2122200‑2122389                                                                                                 |                                                                                              aCATTTCGCCTATGTTCGATTTTGTTTGTAATGCTTGCATATTGTTTGTGCTCCTTTCTGCTATACTCCTATTAAGGAGGTGAATGACTTATGACTGAtc                                                                                            <  1:2946425/100‑2 (MQ=255) aCATTTCGCCTATGTTCGATTTTGTTTGTAATGCTTGCATATTGTTTGTGCTCCTTTCTGCTATACTCCTATTAAGGAGGTGAATGACTTATGACTGATg                                                                                            <  1:5578622/100‑1 (MQ=255)              gTTCGATTTTGTTTGTAATGCTTGCATATTGTTTGTGCTCCTTTCTGCTATACTCCTATTAAGGAGGTGAATGACTTATGACTGATGAAGCTAAATTTgg                                                                               <  1:4469518/100‑2 (MQ=255)                         tttgtAATGCTTGCATATTGTTTGTGCTCCTTTCTGCTATACTCCTATTAAGGAGGTGAATGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAAc                                                                    >  1:5188908/1‑100 (MQ=255)                            gtAATGCTTGCATATTGTTTGTGCTCCTTTCTGCTATACTCCTATTAAGGAGGTGAATGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACttt                                                                 >  1:1399062/1‑100 (MQ=255)                                tGCTTGCATATTGTTTGTGCTCCTTTCTGCTATACTCCTATTAAGGAGGTGAATGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACtttgggg                                                             <  1:1977800/100‑5 (MQ=255)                                    tGCATATTGTTTGTGCTCCTTTCTGCTATACTCCTATTAAGGAGGTGAATGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAAtt                                                         >  1:3157687/1‑100 (MQ=255)                                                            gCTATACTCCTATTAAGGAGGTGAATGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGt                                 >  1:3148944/1‑100 (MQ=255)                                                                             gaggTGAATGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGc                <  1:1357218/100‑1 (MQ=255)                                                                             gaggTGAATGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGc                <  1:2118152/100‑1 (MQ=255)                                                                             gaggTGAATGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGc                <  1:5125706/100‑1 (MQ=255)                                                                              aggTGAATGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCa               <  1:1701878/100‑1 (MQ=255)                                                                                  taaTGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCtt           >  1:1167545/2‑100 (MQ=255)                                                                                  aaaTGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGCATGTCTAAACGTTCTGCATCtt           >  1:2536002/2‑100 (MQ=255)                                                                                   caTGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTa          >  1:118674/2‑100 (MQ=255)                                                                                   caTGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTa          >  1:4554965/2‑100 (MQ=255)                                                                                   aaTGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCAtagat          >  1:3712444/1‑96 (MQ=255)                                                                                    aTGACTTATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTAt         >  1:2178180/1‑100 (MQ=255)                                                                                          tATGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTATTTCGGt   >  1:4209891/1‑100 (MQ=255)                                                                                           aTGACTGATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTATTTCGGTa  >  1:2494830/1‑100 (MQ=255)                                                                                           aTGACTGATGAAGCTAAATTTGTCCTTTTACAACTTCATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTATTTCGGTa  >  1:2661279/1‑100 (MQ=255)                                                                                                 |                                                                                              ACATTTCTCCGATGTTCGAATTTGTTTGTAATGCTTGCATATTGTTTATGCTCCTTTCTGCTATACTCCTATTAAGGAGGTGAATGACTTATGACTAATGAAGCTAAATTTGTCCTTTTACAACTTTATTCAATTTACCTTGATAGAATTGACGAAGGTATGTCTAAACGTTCTGCATCTTATTTCGGTA  >  NC_007793/2122200‑2122389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑