Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,122,111	A→T	D43* (GAT→TAA)	SAUSA300_RS10785 ←	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,122,111	0	A	T	100.0%	15.6 / NA	7	D43E (GAT→GAA)	SAUSA300_RS10785	oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (1/6);  total (1/6)

ATAGCTGTGTAATCTGTATTTTCTTCAAATCCGTATTTAAGCATTCTTGGAAACCAATCTTTATATGCTGTCTTAACTTCTAATGCTTGATGAAGTTCTCGACCGCTGATTGCGATTTCTCCATTTTCTTTTTCTTGAATATTGAAC  >  NC_007793/2122055‑2122201                                                         |                                                                                           gtgactatatAATCGATATTTTCTTCAAATCCGTAATCACTCATTCTTTCAAACCATTTTTTGTATGGAGTCTTAACCTCTAATGCTTGATGAAGTtctc                                                 >  1:2757192/10‑100 (MQ=255)         atAATCGATATTTTCTTCAAATCCGTAATCACTCATTCTTTCAAACCATTTTTTGTATGGAGTCTTAACCTCTAATGCTTGATGAAGTTCTCGACCGCTg                                         <  1:293811/99‑1 (MQ=255)            atcgatATTTTCTTCAAATCCGTAATCACTCATTCTTTCAAACCATTTTTTGTATGGAGTCTTAACCTCTAATGCTTGATGAAGTTCTCGACCGCTGAtt                                      <  1:1777367/95‑1 (MQ=255)              cgatATTTTCTTCAAATCCGTAATCACTCATTCTTTCAAACCATTTTTTGTATGGAGTCTTAACCTCTAATGCTTGATGAAGTTCTCGACCGCTGATTGc                                    <  1:5021357/97‑1 (MQ=255)                 tATTTTCTTCAAATCCGTACTATCTCATTCTTTCAAACCATTTTTTGTATGGAGTCTTAACCTCTAATGCTTGATGAAGTTCTCGACCGCTGATTGCGAt                                 <  1:2663015/100‑1 (MQ=255)                                            ttCTTTCAAACCATTTTTTGTATGGAGTCTTAACCTCTAATGCTTGATGAAGTTCTCGACCGCTGATTGCGATTTCTCCATTTTCTTTTTCTTGTatgtc      <  1:978643/100‑4 (MQ=255)                                                ttcaaaCCATTTTTTGTATGGAGTCTTAACCTCTAATGCTTGATGAAGTTCTCGACCGCTGATTGCGATTTCTCCATTTTCTTTTTCTTGTTTGTTGAAc  <  1:4750467/97‑1 (MQ=255)                                                         |                                                                                           ATAGCTGTGTAATCTGTATTTTCTTCAAATCCGTATTTAAGCATTCTTGGAAACCAATCTTTATATGCTGTCTTAACTTCTAATGCTTGATGAAGTTCTCGACCGCTGATTGCGATTTCTCCATTTTCTTTTTCTTGAATATTGAAC  >  NC_007793/2122055‑2122201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑