Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,121,001	G→A	H3H (CAC→CAT)	SAUSA300_RS10775 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,121,001	0	G	A	100.0%	23.6 / NA	10	H3H (CAC→CAT)	SAUSA300_RS10775	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/10);  total (0/10)

CCATACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATGTGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTATCTCCTAGTGAA  >  NC_007793/2120905‑2121053                                                                                                 |                                                     ccATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAAtatgt                                                   >  1:1772198/1‑100 (MQ=255) aagaaCAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTCTCAGGTATTGGAACTGTTACGTTTAAAAtatgt                                                   >  1:2540833/5‑100 (MQ=255)    tctggggTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGc                                                <  1:4142774/96‑1 (MQ=255)    tACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGc                                                <  1:5032441/100‑1 (MQ=255)    tACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTCCAGGTATTGGAACTGTTACGTTTAAAATATGTGGc                                                <  1:4082272/100‑1 (MQ=255)      ctgggTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTgggca                                              <  1:4909544/98‑4 (MQ=255)       aGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCAtt                                             <  1:1870316/100‑1 (MQ=255)               aTGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATctt                                     <  1:1516677/100‑1 (MQ=255)                                    catATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAAtgttgt                <  1:2169587/100‑1 (MQ=255)                                     atcttCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATgttgtt               <  1:362871/97‑1 (MQ=255)                                                  gtgATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTAtcaacctaaagt  <  1:1703063/100‑11 (MQ=37)                                                  gtgATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTAtcaacctaaagt  <  1:2093567/100‑11 (MQ=37)                                                                                                 |                                                     CCATACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATGTGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTATCTCCTAGTGAA  >  NC_007793/2120905‑2121053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑