Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,120,962	T→C	V16V (GTA→GTG)	SAUSA300_RS10775 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,120,962	0	T	C	100.0%	43.3 / NA	17	V16V (GTA→GTG)	SAUSA300_RS10775	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/15);  total (2/15)

GATGCAATTTTTAATTTCTTCTTTAAGTCACTCATGTTCCATACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATGTGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTATCTCCTAGTGAA  >  NC_007793/2120867‑2121053                                                                                                |                                                                                            gATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGcacac                                                                                         <  1:1688688/100‑2 (MQ=255)  aTGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCAcgta                                                                                        <  1:1357501/100‑4 (MQ=255)     cAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACgtgtgt                                                                                     <  1:5038316/100‑1 (MQ=255)        tttttAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTc                                                                                  <  1:2955525/100‑1 (MQ=255)                    tcttTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTggcat                                                                      <  1:2564429/100‑4 (MQ=255)                      tttAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATGAGCACGTGTGTTTCAGGTATTGGAACta                                                                    <  1:5756938/100‑2 (MQ=255)                                       ccATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAAtatgt                                                   >  1:1772198/1‑100 (MQ=255)                                       aagaaCAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTCTCAGGTATTGGAACTGTTACGTTTAAAAtatgt                                                   >  1:2540833/5‑100 (MQ=255)                                          tctggggTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGc                                                <  1:4142774/96‑1 (MQ=255)                                          tACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGc                                                <  1:5032441/100‑1 (MQ=255)                                          tACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTCCAGGTATTGGAACTGTTACGTTTAAAATATGTGGc                                                <  1:4082272/100‑1 (MQ=255)                                            ctgggTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTgggca                                              <  1:4909544/98‑4 (MQ=255)                                             aGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCAtt                                             <  1:1870316/100‑1 (MQ=255)                                                     aTGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATctt                                     <  1:1516677/100‑1 (MQ=255)                                                                          catATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAAtgttgt                <  1:2169587/100‑1 (MQ=255)                                                                           atcttCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATgttgtt               <  1:362871/97‑1 (MQ=255)                                                                                        gtgATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTAtcaacctaaagt  <  1:2093567/100‑11 (MQ=37)                                                                                        gtgATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTAtcaacctaaagt  <  1:1703063/100‑11 (MQ=37)                                                                                                |                                                                                            GATGCAATTTTTAATTTCTTCTTTAAGTCACTCATGTTCCATACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATGTGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTATCTCCTAGTGAA  >  NC_007793/2120867‑2121053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑