Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,120,938	A→T	D24E (GAT→GAA)	SAUSA300_RS10775 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,120,938	0	A	T	100.0%	43.8 / NA	17	D24E (GAT→GAA)	SAUSA300_RS10775	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (2/15);  total (2/15)

CTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTCACTCATGTTCCATACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATGTGTGGCATTTCTATCTT  >  NC_007793/2120860‑2121018                                                                               |                                                                                 ttcatcaGATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATa                                                             <  1:5484222/99‑1 (MQ=255) ttcatcaGATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATa                                                             <  1:5174785/99‑1 (MQ=255) ttcatcaGATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATa                                                             <  1:1919999/99‑1 (MQ=255)        gATGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGcacac                                                      <  1:1688688/100‑2 (MQ=255)         aTGCAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCAcgta                                                     <  1:1357501/100‑4 (MQ=255)            cAATTTTTAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACgtgtgt                                                  <  1:5038316/100‑1 (MQ=255)               tttttAATTTCTTCTTTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTc                                               <  1:2955525/100‑1 (MQ=255)                           tcttTAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTggcat                                   <  1:2564429/100‑4 (MQ=255)                             tttAAGTCGCTCATGTTCCATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATGAGCACGTGTGTTTCAGGTATTGGAACta                                 <  1:5756938/100‑2 (MQ=255)                                              ccATACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAAtatgt                >  1:1772198/1‑100 (MQ=255)                                              aagaaCAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTCTCAGGTATTGGAACTGTTACGTTTAAAAtatgt                >  1:2540833/5‑100 (MQ=255)                                                 tctggggTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGc             <  1:4142774/96‑1 (MQ=255)                                                 tACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGc             <  1:5032441/100‑1 (MQ=255)                                                 tACAGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTCCAGGTATTGGAACTGTTACGTTTAAAATATGTGGc             <  1:4082272/100‑1 (MQ=255)                                                   ctgggTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTgggca           <  1:4909544/98‑4 (MQ=255)                                                    aGGGTCTAATGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCAtt          <  1:1870316/100‑1 (MQ=255)                                                            aTGAGTAAGCTATTAACTCTTCATATTCATCTTTTGTGATAAGCACGTGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATctt  <  1:1516677/100‑1 (MQ=255)                                                                               |                                                                                 CTCATCAGATGCAATTTTTAATTTCTTCTTTAAGTCACTCATGTTCCATACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATGTGTGGCATTTCTATCTT  >  NC_007793/2120860‑2121018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑